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Instrument to determine the haemoglobin-oxygen equilibrium curve based on the calculated diffusion of oxygen across a semi-permeable membrane

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Abstract

A technique is presented for automatically obtaining the haemoglobin-oxygen equilibrium curve from samples of concentrated haemoglobin solutions or whole blood The amount of oxygen exchanged to the sample across a silicone rubber membrane is calculated from the diffusion equation using a calibrated gas source and the measured pO2 of the sample. With this system it is possible to calculate the entire equilibrium curve from a set of pO2 measurements made as the desaturated sample is raised to an oxygen partial pressure of 150 mm Hg or greater if desired.

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Abbreviations

P * :

Partial pressure of oxygen on gas side of membrane

P(t) :

Measured partial pressure of oxygen in sample as a function of time

MO 2 :

Total quantity of oxygen transferred

b :

Membrane equilibrium time constant

K :

Volume solubility coefficient

References

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Authors and Affiliations

  1. Biomedical Engineering and Instrumentation Branch, Division of Research Services, National Institutes of Health, 20205, Bethesda, Maryand, USA

    L. E. Thibault, W. H. Schuette & D. E. Lees

  2. Anaesthesiology Section, Clinical Centre, National Institutes of Health, 20205, Bethesda, Maryland, USA

    L. E. Thibault, W. H. Schuette & D. E. Lees

Authors
  1. L. E. Thibault
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  2. W. H. Schuette
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  3. D. E. Lees
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Thibault, L.E., Schuette, W.H. & Lees, D.E. Instrument to determine the haemoglobin-oxygen equilibrium curve based on the calculated diffusion of oxygen across a semi-permeable membrane. Med. Biol. Eng. Comput. 18, 401–405 (1980). https://doi.org/10.1007/BF02443308

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  • DOI: https://doi.org/10.1007/BF02443308

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