Abstract
A test system for detecting cytotoxic effects of bioactive substances based on human fibroblast culture is proposed. The effects of acrylamide, streptomycin, cycloheximide, sodium dodecyl sulfate, sanguiritrine, and ethanol were evaluated by organic stain binding. Typical dose-effect relationships were detected for all substances except cycloheximide. The proposed test system can be used for screening of bioactive substances in preclinical trials.
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 5, pp. 587–590, May, 2000
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Chernikov, V.G., Shishkin, S.S., Krokhina, T.B. et al. Use of cultured human fibroblasts for rapid evaluation of cytotoxic effects of bioactive substances. Bull Exp Biol Med 129, 499–501 (2000). https://doi.org/10.1007/BF02439814
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DOI: https://doi.org/10.1007/BF02439814