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Dietary fat saturation, mortality and heart and lung morphology of rats

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Abstract

Rats of both sexes were fed diets containing 21% (wt) fat as beef tallow (BT), safflower oil (SO), or a 60:40 BT:SO mix (M). Some of each group were killed at 9, 12, 15, 18, and 21 months of age. Death by 15 months was significantly (P<.001) greater in SO rats than in other groups. Mortality in SO females was significantly (P<.005) greater than in SO males. A trend for longer life existed in M compared to BT rats. Platelet aggregation microscopically visible in the alveoli of SO males and thrombi in the pulmonary arteries of SO females suggest that death in these animals may have been related to dietary linoleate. Excessive production of prostaglandins may have induced platelet aggregation and possibly interfered with normal immune responses. Myocardial stellate scars were seen earlier in M males than in other groups. Areas of massive infarction and subsequent fibrosis of the heart were present in BT rats and M males at 15 months but not until 18 months in M females.

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References

  1. Report of the Inter-Society Commission for Heart Disease Resources Primary prevention of atherosclerotic disease. Cir., 42:A55–A95, 1970.

    Google Scholar 

  2. Miettinen, M., Turpeinen, O., Karvonen, M. J., Elosuo, R. and Paavilainen, E.: Effect of cholesterol lowering diet on mortality from coronary heart disease and other causes-12-yr clinical trial in men and women. Lancet, 2:835–838, 1972.

    Article  PubMed  CAS  Google Scholar 

  3. Harman, D.: Free-radical theory of aging-effect of amount and degree of unsaturation of dietary fat on mortality rate. J. Geront., 26:451–457, 1971.

    PubMed  CAS  Google Scholar 

  4. Dupont, J., Mathias, M. M. and Cabacungan, N. B.: Dietary lipid, fatty acid synthesis and cholesterol metabolism in aging rats. Lipids, 9: 576–589, 1972.

    Google Scholar 

  5. Kaunitz, H. and Johnson, R. E.: Exacerbation of heart and liver lesions in rats by feeding various mildly oxidized fats. Lipids, 8:329–336, 1973.

    PubMed  CAS  Google Scholar 

  6. Wicks, M. S., Ball, C. R. and Williams, W. L.: Relation of types of dietary fat to cardiovascular damage in mice. Am. J. Anat., 124:481–489, 1967.

    Article  Google Scholar 

  7. Scott, R. F., Imai, H., Goodale, F., Lee, K. T. and Morrison, E.: Dietary induced myocardial infarction in rats: the possible role of essential fatty acid deficiency. Exp. Mol. Path., 1:1–14, 1962.

    Article  CAS  Google Scholar 

  8. Bouchardy, B. and Majno, G.: Histopathology of early myocardial infarcts. Am. J. Path., 74:301–317, 1974.

    PubMed  CAS  Google Scholar 

  9. Lie, J. T., Holley, K. E., Kampa, W. R. and Titus, J. L.: New histochemical diagnosis of early stages of myocardial ischemia. Mayo Clin. Proc., 46:319–327, 1971.

    PubMed  CAS  Google Scholar 

  10. Mantel, N.: Evaluation of survival data and two new rank order statistics arising in its consideration. Can. Chem. Repts., 50:163–170, 1966.

    CAS  Google Scholar 

  11. Berg, B. N. and Harmison, C. R.: Growth, disease and aging in the rat. J. Geron., 12:370–377, 1957.

    CAS  Google Scholar 

  12. Habermann, R. T., Williams, F. P., Jr., McPherson, C. W. and Every, R. R.: The effect of orally administered sulfamerazine and cholorotetracycline on chronic respiratory disease in rats. Lab. An. Care, 13: 28–40, 1963.

    CAS  Google Scholar 

  13. Hunter, C., Barer, G. R., Shaw, J. W. and Clegg, E. J.: Growth of the heart and lungs in hypoxic rodents: a model for human hypoxic disease. App. Sci. Mol. Med., 46:375–391, 1974.

    CAS  Google Scholar 

  14. Harman, D., Hendricks, S., Eddy, D. E. and Seibold, J.: Free radical theory of aging: effect of dietary fat on central nervous system function. J. Am. Ger. Soc., 24:301–307, 1976.

    CAS  Google Scholar 

  15. Gluck, L. and Kulovish, M. V.: Fetal lung development. Ped. Clin. N. Am., 20:367–379, 1973.

    CAS  Google Scholar 

  16. Dupont, J., Spindler, A. A. and Mathias, M. M.: Dietary fat saturation and cholesterol and fatty acid synthesis in aging rats. Age, 1: 93–99, 1978.

    CAS  Google Scholar 

  17. Pirkle, H. and Carstens, P.: Pulmonary platelet aggregates associated with sudden death in man. Science, 185:1062–1064, 1974.

    PubMed  CAS  Google Scholar 

  18. Silver, M. J., Hoch, W., Kocsis, J. J., Ingerman, C. M. and Smith, J. B.: Arachidonic acid causes sudden death in rabbits. Science, 183:1085–1087, 1974.

    PubMed  CAS  Google Scholar 

  19. Bakhle, Y. S. and Vane, J. R.: Pharmacokinetic function of the pulmonary circulation. Physiol. Rev., 54:1007–1045, 1974.

    PubMed  CAS  Google Scholar 

  20. Kadowitz, P. J., Joiner, P. D. and Hyman, A. L.: Physiological and Pharmacological Roles of Prostaglandins. Ann. Rev. Pharm., 15:285–306, 1975.

    Article  CAS  Google Scholar 

  21. Mathe, A. A., Hedquist P., Strandberg, K. and Leslie, C. A.: Aspects of Prostaglandin Function in the Lung. N. Engl. J. Med., 296:850–855; 910–914, 1977.

    Article  PubMed  CAS  Google Scholar 

  22. Hwang, D. H., Mathias, M. M. and Dupont, J.: Linoleate enrichment of diet and prostaglandin metabolism. J. Nutr., 105:995–1002, 1975.

    PubMed  CAS  Google Scholar 

  23. Machlin, L. J.: Vitamin E and prostaglandins (PG), in Tocopherol, Oxygen and Biomembranes, edited by C. de Duve and O. Hayaishi, Elsevier North Holland Biomedical Press, New York, 1978, 179–189.

    Google Scholar 

  24. Tengerdy, R. P., Heinzerling, R. H. and Mathias, M. M.: Effect of vitamin E on disease-resistance and immune responses, in Tocopherol, Oxygen and Biomembranes, edited by C. de Duve and O. Hayaishi, Elsevier North Holland Biomedical Press, New York, 1978, 191–200.

    Google Scholar 

  25. Harman, D., Heidrick, M. L and Eddy, D. E. Free radical theory of aging: effect of free-radical-reaction inhibitors on the immune response. J. Am. Geriatrics Soc., 25:400–407, 1977.

    CAS  Google Scholar 

  26. Consumer and Food Economic Research Division, ARS, USDA. Food intake and nutritive value of diets of men, women and children in the United States, Spring, 1965. U.S. Government Printing Office, Washington, D.C., p. 21, 1969.

    Google Scholar 

  27. Committee on Animal Nutrition, National Academy of Sciences-National Research Council, Nutrient Requirements of Domestic Animals. X. Nutrient Requirements of Laboratory Animals. Washington, D.C., Publication 990, 1962.

  28. Humason, G. Animal Tissue Techniques. 2nd edition, San Francisco, W. H. Freeman and Company, 1967.

    Google Scholar 

  29. Hayat, M. A. Principles and Techniques of Electron Microscopy. Biological Applications. New York, Van Nostrand Reinhold Company, 1970.

    Google Scholar 

  30. Trump, B. F., Smuckler, E. A. and Benditt, E. P. A method for staining epoxy sections for light microscopy. J. Ultrastructure Res. 5:343–348, 1961.

    Article  CAS  Google Scholar 

  31. Folch, J., Lees, M. and Sloane-Stanley, G. H. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226:497–509, 1957.

    PubMed  CAS  Google Scholar 

  32. Morrison, W. R. and Smith, L. M. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res., 5:600–601, 1964.

    PubMed  CAS  Google Scholar 

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Author notes

  1. Audrey A. Spindler

    Present address: School of Family Studies and Consumer Sciences, San Diego State College, San Diego, California

  2. Jacqueline Dupont

    Present address: Department of Food and Nutrition, Iowa State University, Ames, Iowa

Authors and Affiliations

  1. Department of Food Science and Nutrition, Colorado State University, Fort Collins, Colorado, 80523

    Audrey A. Spindler, Jacqueline Dupont & Melvin M. Mathias

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  1. Audrey A. Spindler
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  2. Jacqueline Dupont
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  3. Melvin M. Mathias
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Spindler, A.A., Dupont, J. & Mathias, M.M. Dietary fat saturation, mortality and heart and lung morphology of rats. AGE 1, 85–92 (1978). https://doi.org/10.1007/BF02432103

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