Skip to main content
SpringerLink
Log in

Genetic and physical characterization of theLR1 leaf rust resistance locus in wheat (Triticum aestivum L.)

  • Original Paper
  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Abstract

The objective of this study was to characterize the leaf rust resistance locusLr1 in wheat. Restriction fragment length polymorphism (RELP) analysis was performed on the resistant lineLr1/6*Thatcher and the susceptible varieties Thatcher and Frisal, as well as on the segregating F2 populations. Seventeen out of 37 RFLP probes mapping to group 5 chromosomes showed polymorphism betweenLr1/6*Thatcher and Frisal, whereas 11 probes were polymorphic between the near-isogenic lines (NILs)Lr1/6*Thatcher and Thatcher. Three of these probes were linked to the resistance gene in the segregating F2 populations. One probe (pTAG621) showed very tight linkage toLr1 and mapped to a single-copy region on chromosome 5D. The map location of pTAG621 at the end of the long arm of chromosome 5D was confirmed by the absence of the band in the nulli-tetrasomic line N5DT5B of Chinese Spring and a set of deletion lines of Chinese Spring lacking the distal part of 5DL. Twenty-seven breeding lines containing theLr1 resistance gene in different genetic backgrounds showed the same band asLr1/6*Thatcher when hybridized with pTAG621. The RFLP marker was converted to a sequence-tagged-site marker using polymerase chain reaction (PCR) amplification. Sequencing of the specific fragment amplified from both NILs revealed point mutations as well as small insertion/deletion events. These were used to design primers that allowed amplification of a specific product only from the resistant lineLr1/6*Thatcher. This STS, specific for theLr1 resistance gene, will allow efficient selection for the disease resistance gene in wheat breeding programmes. In addition, the identification of a D-genome-specific probe tightly linked toLr1 should ultimately provide the basis for positional cloning of the gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ausemus ER, Harrington JB, Reitz LP, Worzella WW (1946) A summary of genetic studies in hexaploid and tetraploid wheats. J Am Soc Agron 38:1082–1099

    Google Scholar 

  • Beckmann JS, Soller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet 67:35–43

    Article  Google Scholar 

  • Browder LE (1980) A compendium of information about named genes for low reaction toPuccinia recondita in wheat. Crop Sci 20:775–779

    Article  Google Scholar 

  • Casulli FM, Pasquini A, Siniscalo A (1988) Virulence of wheat leaf rust in Italy. In: Zwatz B (ed) Proceedings 7th European and Mediterranean Cereal Rusts Conference, Vienna, pp 127–129

  • Causse MA, Fulton TM, Cho YG, Ahn SN, Chungwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksley SD (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251–1274

    CAS  PubMed  Google Scholar 

  • Cheung WY, Moore G, Money TA, Gale MD (1992)HapII library indicates methylation-free islands in wheat and barley. Theor Appl Genet 84:739–746

    Article  Google Scholar 

  • Devos KM, Gale MD (1992a) The genetic maps of wheat and their potential in plant breeding. Outlook on Agriculture 22:93–99

    Google Scholar 

  • Devos KM, Gale MD (1992b) The use of randomly amplified DNA markers in wheat. theor Appl Genet 84:567–572

    Article  Google Scholar 

  • D'Ovidio R, Tanzarella OA, Porceddu E (1990) Rapid and efficient detection of genetic polymorphisms in wheat through amplification by the polymerase chain reaction. Plant Mol Biol 15:169–171

    Article  PubMed  Google Scholar 

  • Dyck PL, Samborski DJ (1968) Genetics of resistance to leaf rust in common wheat varieties Webster, Loros, Brevit, Carina, Malakoff and Centenario. Can J Genet Cytol 10:7–17

    Google Scholar 

  • Eastwood RF, Lagudah ES, Appels R (1994) A direct search for DNA sequences tightly linked to cereal cyst nematode resistance genes inTriticum tauschii. Genome 37:311–319

    CAS  PubMed  Google Scholar 

  • Endo TR (1988) Induction of chromosomal structural changes by a chromosome ofAegilops cylindrica L. in common wheat. J Hered 79:366–370

    Google Scholar 

  • Gill BS (1991) The utility and basis of chromosome banding methods. In: Kimber G (ed) Proceedings International Symposium Chromosome Engineering in Plants. Columbia, Mo., 13–15 August 1990. University of Missouri, Columbia

    Google Scholar 

  • Gill BS, Friebe B, Endo TR (1991) Standard karyotype and nomenclature system for description of chromosome bands and structural-aberrations in wheat (Triticum aestivum). Genome 34:830–839

    Google Scholar 

  • Gill KS, Gill BS, Endo TR, Mukai Y (1993) Fine physical mapping ofPh1, a chromosome pairing regulator gene in polyploid wheat. Genetics 134:1231–1236

    CAS  PubMed  Google Scholar 

  • Graner A, Siedler H, Jahoor A, Herrmann RG, Wenzel G (1990) Assessment of the degree and type of polymorphism in barley (Hordeum vulgare). Theor Appl Genet 80:826–832

    Article  Google Scholar 

  • Johnson R, Lupton FGH (1987) Breeding for disease resistance. In: Lupton FGH (ed) Wheat breeding. Its scientific basis. Chapman and Hall, Cambridge, pp 369–418

    Google Scholar 

  • Jones DA, Thomas CM, Hammond-Kosack KE, Balint-Kurti PJ, Jones JDG (1994) Isolation of the tomatoCf-9 gene for resistance toCladosporum fulvum by transposon tagging. Science 266:789–793

    CAS  PubMed  Google Scholar 

  • Joosten MHAJ, Cozjinsen TJ, De Witt PJGM (1994) Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulence gene. Nature 367:384–386

    Article  CAS  PubMed  Google Scholar 

  • Kilian A, Steffenson BJ, Kleinhofs A (1995) Towards the cloning of theRPG1 locus in barley via rice? Plant Genome III, Abstracts p 53, January 15–19, San Diego, Calif.

  • Knott DR (1989) The wheat rusts-breeding for resistance. Monographs on Theoretical and Applied Genetics, vol 12. Springer, Berlin

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Kurata N, Moore G, Nagamura Y, Foote T, Yaro M, Minobe Y, Gale M (1994) Conservation of genome structure between rice and wheat. Bio/technology 12:276–278

    Article  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamsom J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Liu YG, Tsunewaki K (1991) Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn J Genet 66:617–633

    CAS  PubMed  Google Scholar 

  • Long DL, Roelfs AP, Leonard KJ, Roberts JJ (1993) Virulence and diversity ofPuccinia recondita f. sp.tritici in United States in 1991. Plant Dis 77:786–791

    Article  Google Scholar 

  • Mains EB, Jackson HS (1926) Physiologic specilization in leaf rust of wheat,Puccinia recondita Erikss. Phytopathology 16:89–120

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Martin GB, Brommonschenckel SH, Chungwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993a) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436

    CAS  PubMed  Google Scholar 

  • Martin GB, De Vicente C, Tanksley SD (1993b) High resolution linkage analysis and physical characterization of the Pto bacterial resistance locus in tomato. Mol Plant-Microb Interact 6:26–34

    CAS  Google Scholar 

  • McIntosh RA (1988) Catalogue of gene symbols for wheat. In: Miller TE, Koeber RMD (eds) Proceedings of 7th International Wheat Genetic Symposium, Cambridge, p 1273

  • McIntosh RA, Baker EP (1970) Cytogenetical studies in wheat. IV. Chromosome location and linkage studies involving thePm2 locus for powdery mildew resistance. Euphytica 19:71–77

    Article  Google Scholar 

  • McIntosh RA, baker EP, Driscoll CJ (1965) Cytogenetical studies in wheat. I. Monosomic analysis of leaf rust resistance in the cultivars Uruguay and Transfer. Aust J Biol Sci 18:971–977

    Google Scholar 

  • Mindrinos M, Katagari F, Yu GL, Ausubel FM (1994) TheA. thaliana disease resistance geneRPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell 78:1089–1099

    Article  CAS  PubMed  Google Scholar 

  • Olson M, Hood L, Cantor CH, Botstein D (1989) A common language for physical mapping of the human genome. Science 24:1434–1435

    Google Scholar 

  • Perez de la Vega M (1993) Biochemical characterization of populations. In: Hayward MD, Bosemark NO, Romagosa I (eds) Plant breeding, principles and prospects. Chapman and Hall, London, pp 184–197

    Google Scholar 

  • Riede CR, Fairbanks DJ, Andersen WR, Kehrer RL, Robison (1994) Enhancement of RAPD analysis by restriction-endonuclease digestion of template DNA in wheat. Plant Breeding 113:254–257

    Article  CAS  Google Scholar 

  • Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico City

    Google Scholar 

  • Ronald PC, Albano B, Tabien R, Abenes L, Wu K, McCouch S, Tanksley SD (1992) Genetic and physical analysis of the rice bacterial blight disease resistance locus,Xa21. Mol Gen Genet 236:113–120

    CAS  PubMed  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    CAS  PubMed  Google Scholar 

  • Schachermayr G, Siedler H, Gale MD, Winzeler H, Winzeler M, Keller B (1994) Identification and localization of molecular markers linked to theLr9 leaf rust resistance gene of wheat. Theor Appl Genet 88:110–115

    Article  CAS  Google Scholar 

  • Schachermayr GM, Messmer MM, Feuillet C, Winzeler H, Winzeler M, Keller B (1995) Identification of molecular markers linked to theAgropyrum elongatum-derived leaf rust resistance geneLr24 in wheat. Theor Appl Genet 30:982–990

    Google Scholar 

  • Sears ER (1966) Chromosome mapping with the aid of telocentrics, In: MacKey J (ed) Proceedings 2nd International Wheat Genetics Symposium (Hereditas Suppl 2) Lund, pp 370–381

  • Siedler H, Messmer MM, Schachermayr GM, Winzeler H, Winzeler M, Keller B (1994) Genetic diversity in European wheat and spelt breeding material based on RFLP data. Theor Appl Genet 88:994–1003

    Article  Google Scholar 

  • Singh RP, Rajaram S (1991) Genetics of adult-plant resistance of leaf rust ‘Frontana’ and three CIMMYT wheats. Genome 35:24–31

    Google Scholar 

  • Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794

    Article  CAS  Google Scholar 

  • Tingey S, Del Tufo JP (1993) Genetic analysis with random polymorphic DNA markers. Plant Physiol 101:349–352

    Article  CAS  PubMed  Google Scholar 

  • Tragoonrung S, Kanazin V, Hayes PM, Blake TK (1992) Sequencetagged-site-facilitated PCR for barley genome mapping. Theor Appl Genet 84:1002–1008

    Article  CAS  Google Scholar 

  • Von Kröcher C, Bartels G, Fehrmann H (1992) Studies on the physiological specilization of leaf rust in wheat (Puccinia recondita Rob. ex Desm. f. sp.tritici Eriks. & Henn). J Plant Dis Prot 99:137–144

    Google Scholar 

  • Waugh R, Powell W (1992) Using RAPD markers for crop improvement. Trends Biotech 10:186–191

    Article  CAS  Google Scholar 

  • Weeks JT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077–1084

    CAS  PubMed  Google Scholar 

  • Weining S, Langridge P (1991) Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theor Appl Genet 82:209–216

    Article  CAS  Google Scholar 

  • Werner JE, Endo TR, Gill BS (1992) Towards a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311

    CAS  PubMed  Google Scholar 

  • Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance geneN: similarity to Toll and interleukine-1 receptor. Cell 78:1101–1115

    Article  CAS  PubMed  Google Scholar 

  • Williams GK, Kubelik AR, Kenneth JL, Rafalski A, Scott VT (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic marker. Nucleic Acids Res 18:6531–6565

    CAS  PubMed  Google Scholar 

  • Williams MNV, Pande N, Nair S, Mohan M, Bennet J (1991) Restriction fragment length polymorphism analysis of polymerase chain products amplified from mapped loci of rice (Oryza sativa L.) genomic DNA. Theor Appl Genet 82:489–498

    Article  CAS  Google Scholar 

  • Winzeler M, Winzeler H, Keller B (1995) Endopeptidase polymorphism and linkage of theEp-D1c null allele with theLr19 leaf rust resistance gene in hexaploid wheat. Plant Breeding 114:24–28

    Article  CAS  Google Scholar 

  • Xie DX, Devos K, Moore G, Gale M (1993) RFLP-based maps of the homoeologous group 5 chromosomes of bread wheat (Triticum aestivum L.). Theor Appl Genet 87:70–74

    Article  CAS  Google Scholar 

  • Yamamori M, Nakamura T, Endo TR, Nagamine T (1994) Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theor Appl Genet 89:179–184

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Breeding, Swiss Federal Research Station for Agronomy, FAP Reckenholz, Reckenholzstrasse 191, 8046, Zürich, Switzerland

    Catherine Feuillet, Monika Messmer, Gabriele Schachermayr & Beat Keller

Authors
  1. Catherine Feuillet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Monika Messmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gabriele Schachermayr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Beat Keller
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Saedler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feuillet, C., Messmer, M., Schachermayr, G. et al. Genetic and physical characterization of theLR1 leaf rust resistance locus in wheat (Triticum aestivum L.). Molec. Gen. Genet. 248, 553–562 (1995). https://doi.org/10.1007/BF02423451

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02423451

Key words

Search

Navigation