Skip to main content
SpringerLink
Log in

Utilisation du15N2 pour estimer la fixation d'azote et sa repartition chez les legumineuses

Use of15N to estimate dinitrogen fixation and distribution in legumes

  • Published:
Plant and Soil Aims and scope Submit manuscript

Resumé

Les auteurs proposent une méthode d'utilisation du15N2 pour l'étude de la fixation d'azote dans les associations complexes légumineuses-Rhizobium cultivées sur sol. La procédure consiste, à marquer l'atmosphère du sol à l'aide de15N2 et à calculer la quantité d'azote total fixé pendant cette période. Les premiers résultats obtenus sur des haricots et des trèfles démontrent qu'après seulement 7 h d'incubation, des quantités significatives de15N sont mesurées dans les plantes permettant ainsi de déterminer précisément la valeur du rapport C2H4/N2 qui a été établi entre 2,6 et 3,1 dans les conditions de l'expérience. Sur de longues périodes, ces mêmes quantités se sont avérées suffisantes pour suivre la dynamique de l'azote des nodules vers les organes reproducteurs de plants de haricots.

Summary

A method for use of15N2 in the study of dinitrogen fixation by complex legumes-Rhizobium associations grown on soil is proposed. The procedure consists in labelling the soil atmosphere with15N2 during short periods of time, measuring15N enrichment in the plants and calculating the total nitrogen fixed during this period. The first results obtained with bean and clover plants demonstrate that after only 7 h of incubation, significative amounts of15N are measured in the plants to allow precise determination of C2H4/N2 ratio which ranged between 2.6 and 3.1 in this experiment. Over longer periods of time, such amounts are meaningfull to follow the pattern of N dynamic from the nodules to the reproductive organs of bean plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bergersen, F. J. 1962 The effects of partial pressure of oxygen upon respiration and nitrogen fixation by soybean root nodules. J. Gen. Microbiol.29, 113–125.

    CAS  Google Scholar 

  2. Bergersen, F. J. 1970 The quantitative relationship between nitrogen fixation and the acetylene-reduction assay. Aust. J. Biol. Sci.23, 1015–1025.

    CAS  Google Scholar 

  3. Bremner, J. M. 1965 Total nitrogenIn Methods of Soil Analysis. Agron.9. Am. Soc. Agron. Madison USA, pp 1149–1176.

    Google Scholar 

  4. Burris, R. H. 1974 MethodologyIn The Biology of Nitrogen Fixation. Ed. A. Quispel. North Holland Publishing co. pp 19–33.

  5. Burris, R. H. and Miller, C. E. 1941 Science93, 114–115.

    CAS  Google Scholar 

  6. Dilworth, M. J. 1966 Acetylene reduction by nitrogen fixing preparations fromClostridium pasteurianum. Biochim. Biophys. Acta127, 285–294.

    CAS  PubMed  Google Scholar 

  7. Hardy, R. W. F., Burns, R. C. and Holsten, R. D. 1973 Applications of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol. Biochem.5, 47–81.

    Article  CAS  Google Scholar 

  8. Hauck, R. D. and Bremner, J. M. 1976 Use of tracers for soil and fertilizer nitrogen research. Adv. Agron.28, 219–266.

    Google Scholar 

  9. Mac Rae, I. C. 1975 Assessment of C2H2∶N2 molar ratios of detachedPhaseolus vulgaris nodules using emission spectrometry fo14N∶15N determinations Soil Biol. Biochem.7, 239–240.

    CAS  Google Scholar 

  10. Martin, A. E. and Ross, P. J. 1968 Significance of errors in15N measurement in soil-plant research. Trans. Int. Congr. Soil Sci. 9th. Adelaide, Australia, Vol3, pp 521–529.

    CAS  Google Scholar 

  11. Oghoghorie, G. G. O. and Pate, J. S. 1972 Exploration in the nitrogen transport system of a nodulated legume using15N. Planta Berlin104, 35–49.

    CAS  Google Scholar 

  12. Saito, S. M. T., Matsui, E. and Salati, E. 198015N2 fixation, H2 evolution and C2H2 reduction relationships inPhaseolus vulgaris. Physiol. Plant.49, 37–42.

    CAS  Google Scholar 

  13. Schollhorn, R. and Burris, R. H. 1966 Study of intermediates in nitrogen fixation. Fed. Proc., Fed. Am. Soc. Exp. Biol.25, 710.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire d'Ecologie, Dpt de Biologie Végétale, Université C. Bernard, Lyon, France

    D. par Montange & R. et Bardin

  2. C. E. P. E. L. Emberger, B.P. 5051, 34033, Montpellier Cedex, France

    F. R. Warembourg

Authors
  1. D. par Montange
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. R. Warembourg
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. et Bardin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

par Montange, D., Warembourg, F.R. & et Bardin, R. Utilisation du15N2 pour estimer la fixation d'azote et sa repartition chez les legumineuses. Plant Soil 63, 131–139 (1981). https://doi.org/10.1007/BF02374592

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02374592

Key Words

Search

Navigation