Summary
The dark (non-photosynthetic) CO2 fixation was studied in the leaves of ammonium-fed and nitratefed tomato plants. The ability to fix14CO2 in the dark of ammonium-fed plants was remarkably lower as compared with nitrate-fed plants, supporting the previous finding that the synthesis of C4-compounds from C3-compounds was reduced in the leaves of ammonium-fed plants. There was no difference in the activity of PEP carboxylase in extracts prepared from the leaves between both the plants during an early period of the treatment. However, the enzyme activity began to decrease rapidly in ammonium-fed plants 4 days after the treatment. By long-term treatments, the enzyme activity in ammonium-fed plants became half as high as that of nitrate-fed plants. The decreased PEP carboxylase activity in ammonium-fed plants was not associated with the presence of NH4-N and the absence of NO3-N in the leaf extract, and was not restored by the addition of the leaf extract from nitrate-fed plants. It is concluded that the decreased rate of synthesis of C4-compounds from C3-compounds in ammonium-fed plants is closely associated with a decrease in the dark fixation involving PEP carboxylase.
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References
Ben-Zioni, A., Vaadia, Y. and Lips, S. H. 1970 Correlation between nitrate reduction, protein synthesis and malate accumulation. Physiol. Plant23, 1039–1047.
Ben-Zioni, A., Vaadia, Y. and Lips, S. H. 1971 Nitrate uptake by roots as regulated by nitrate reduction products of the shoot. Physiol. Plant.24, 288–290.
Breteler, H. 1973 A comparison between ammonium and nitrate nutrition of young sugar-beet plants grown in nutrient solutions at constant acidity. 1. Production of dry matter, ionic balance and chemical composition. Neth. J. Agric. Aci.21, 227–244.
Givan, C. V. 1979 Metabolic detoxification of ammonia in tissues of higher plants. Phytochem.18, 375–382.
Hammel, K. E., Cornwell, K. L. and Bassham, J. A. 1979. Stimulation of dark CO2 fixation by ammonia in isolated mesophyll cells ofPapaver somniferum L. Plant Cell Physiol.20, 1523–1529.
Harada, T., Takaki, H. and Yamada, Y. 1968 Effect of nitrogen sources on the chemical components of young plants. Soil Sci. Plant Nutr.14, 47–55.
Ikeda, M., Yamada, Y. and Harada, T. 1974 Glucose metabolism in detached leaves of tomato plants grown with ammonium and nitrate as nitrogen sources. Soil Sci. Plant Nutr.20, 185–194.
Ikeda, M. and Yamada, Y. 1978 Incorporation of inorganic nitrogen into protein fraction in tomato plants grown with ammonium and nitrate as nitrogen sources. J. Fac. Agric. Kyushu Univ.22, 161–168.
Jackson, W. A. and Coleman, N. T. 1959 Fixation of carbon dioxide by plant roots through phosphoenolpyruvate carboxylase. Plant and Soil11, 1–16.
Kirkby, E. A. 1968 Influence of ammonium and nitrate nutrition on the cation-anion balance and nitrogen and carbohydrate metabolism of white mustard plants grown in dilute nutrient solutions. Soil Sci.105, 133–141.
Matsumoto, H., Wakiuchi, N. and Takahashi, E. 1971 Changes of some mitochondrial enzyme activities of cucumber plants during ammonium toxicity. Physiol. Plant.25, 353–357.
Merkel, D. 1973 Der Einfluss des NO3∶NH4-Verhältnisses in der Nährlösung auf Ertrag und Gehalte an organischen und anorganischen Ionen von Tomatenpflanzen. Z. Pflanzenernaehr. Bodenkd.134, 236–246.
Miyachi, S., Kamiya, A. and Miyachi, S. 1977 Wavelength effects of incident light on carbon metabolism inChlorella cells. In Biological Solar Energy Conversion. Academic Press, N.Y. pp 167–182.
Paul, J.S., Cornwell, K.L. and Bassham, J.A. 1980 Effects of ammonia on carbon metabolism in photosynthesizing isolated mesophyll cells fromPapaver somniferum L. Planta142, 49–54.
Peak, J. G., Peak, M. J. and Ting, I. P. 1980 Heterotrophic carbon dioxide fixation products ofEuglena. Effects of ammonium. Plant Physiol.65, 566–568.
Raven, J. A. and Smith, F. A. 1976 Nitrogen assimilation and transport in vascular land plants in relation to intracellular pH regulation. New Phytol.76, 415–431.
Smith, F. A. and Raven, J. A. 1979 Intracellular pH and its regulation. Annu. Rev. Plant Physiol.30, 289–311.
Splittsoesser, W. E. 1966 Dark CO2 fixation and its role in the growth of plant tissues. Plant Physiol.41, 755–759.
Stuart, D. M. and Haddock, J. L. 1968 Inhibition of water uptake in sugar beet roots by ammonia. Plant Physiol.43, 345–350.
Thomas, G. W., Coleman, N. T. and Jackson, W. A. 1959 Influence of magnesium, potassium, and nitrogen nutrition on phosphoenolpyruvate-stimulated carbon dioxide fixation. Agron. J.51, 591–594.
Vickery, H. B., Pucher, G. W., Wakeman, A. J. and Leavenworth C. S. 1940 Chemical investigation of the tobacco plant. VIII. The effect upon the composition of the tobacco plant of the form in which nitrogen is supplied. Bull. Conn. Agric. Stn.442, 65–119.
Vines, H. M. and Wedding, R. T. 1960 Some effects of ammonia on plant metabolism and a possible mechanism for ammonia toxicity. Plant Physiol.35, 820–825.
Wakiuchi, N., Matsumoto, H. and Takahashi, E. 1971 Changes of some enzyme activities of cucumber during ammonium toxicity Physiol. Plant.24, 248–253.
Wooley, J. T., Hicks, G. P. and Hageman, R. H. 1960 Rapid determination of nitrate and nitrite in plant material. J. Agric. Food Chem.8, 481–482.
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Ikeda, M., Yamada, Y. Dark CO2 fixation in leaves of tomato plants grown with ammonium and nitrate as nitrogen sources. Plant Soil 60, 213–222 (1981). https://doi.org/10.1007/BF02374106
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DOI: https://doi.org/10.1007/BF02374106