Abstract
Trois Baculovirus isolés de larves deSpodoptera littoralis atteintes de polyédroses nucléaires au Maroc, en Egypte et dans un élevage à Lyon ont été comparés par analyse de leurs génomes avec des enzymes de restriction. Les variants génétiques contenus dans les populations virales sont obtenus après isolement de clones en culture cellulaire. Sur 27 clones analysés par les endonucléases on observe 11 variants avecEco RI, 3 variants avecBam HI et avecSac I et un type unique avecHin dIII. Les 3 virus Maroc, Egypte et Lyon sont différents, mais appartiennent à l'espèce B définie parCherry & Summers (1985).
Summary
Three Baculoviruses were isolated fromSpodoptera littoralis larvae infected with nuclear polyhedrosis (NPV) collected from Morocco, Egypt, and a from laboratory culture at Lyon (France). Their genomes were compared by restriction endonuclease analysis. The genotypic variants contained in the viral populations were obtained after isolation of clones in cell culture. Among the 27 clones analyzed by restriction endonucleases, 11 variants were observed withEco RI, 3 variants withBam HI andSac I, and one unique type withHin dIII. These 3 viruses “Morocco”, “Egypt”, and “Lyon” are different, but belong to type B ofS. littoralis NPV as defined byCherry & Summers (1985).
This is a preview of subscription content, log in via an institution to check access.
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
References
Abul-Nasr, S. — 1956. Polyhedrosis virus disease on cotton leaf-wormProdenia litura F. —Bull. Soc. Entomol. (Egypte), 40, 321–332.
Burgess, S. — 1983.Eco RI Restriction endonuclease fragment pattern of eight lepidopteran baculoviruses. —J. Invertebr. Pathol., 42, 401–404.
Cherry, C.L. &Summer, M.D. — 1985. Genotypic variation among wild isolates of two nuclear polyhedrosis viruses isolated fromSpodoptera littoralis. —J. Invertebr. Pathol., 46, 289–295.
Croizier, G., Godse, D. &Vlak, J.—1980. Selection de types viraux dans les infections doubles à baculovirus chez les larves de lépidoptères. —C.R. Acad. Sci. (Paris, Sci. D), 290, 579–582.
Kislev, N. &Edelman, M. — 1982. DNA restriction pattern differences from geographic isolates ofSpodoptera littoralis nuclear polyhedrosis virus. —Virol., 119, 219–222.
McGowan, V.F. & Skerman, V.B.D. ed. — 1982. World directory of collections of cultures of microorganisms. —World date Center, University Queensland, 2nd ed., 641 p.
Merdan, A., Croizier, L., Veyrunes, J.C. &Croizier, G. — 1977. Etude comparée des protéines des polyèdres et des virions de trois isolats de Baculovirus deSpodoptera littoralis. —Entomophaga, 2, 413–420.
Mialhe, E., Quiot, J.M. &Paradis, S. — 1984. Etablissement de deux lignées cellulaires deSpodoptera littoralis [Lep.: Noctuidae] permissives pour des virus susceptibles d'être utilisés en lutte microbiologique. —Entomophaga, 29, 347–350.
Miller, L.K. &Dawes, K.P. — 1978. Restriction endonuclease analysis to distinguish two closely related nuclear polyhedrosis viruses:Autographa californica MNPV andTrichoplusia ni MNPV. —Environ. Microbiol., 35, 1206–1210.
Author information
Authors and Affiliations
Additional information
avec la collaboration technique de BéatriceChebli.
Rights and permissions
About this article
Cite this article
Croizier, G., Quiot, J.M., Paradis, S. et al. Comparaison de la composition génétique de trois isolats du baculovirus de la polyedrose nucléaire du lépidoptèreSpodoptera littoralis . Entomophaga 31, 385–392 (1986). https://doi.org/10.1007/BF02373155
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02373155