Abstract
Samples of a gypsy moth nucleopolyhedrosis virus product, Gypchek®, were taken each day during a 100-day production run and monitored for the presence of pathogenic bacteria and fungi. The standard plate count/g of product was 5.97±1.51×108 over the 100-day period, while the sporulating bacteria count was 3.81±1.21×106/g. We did not detect obligate anaerobic or fecal coliform bacteria in any of the samples.Bacillus cereus, Staphylococcus epidermidis, B. licheniformis, Streptococcus faecalis, Serratia liquefaciens, andAspergillus niger were the most frequently isolated microorganisms. We did not detect primary pathogenic bacteria or fungi, but the presence of opportunistic pathogens indicated that assiduous monitoring of the virus production facility and rigorous quality control of production batches are necessary.
Résumé
Des échantillons d'une préparation à base du virus de la nucléopolyhédrose deLymantria dispar (L.), le Gypchek®, furent prélevés tous les jours durant un cycle de production de 100 jours, et ont été examinés afin d'y détecter des bactéries et champignons pathogénes. Le nombre moyen de colonies bactériennes par gramme du produit était de 5.97±1.51×108 durant la période de 100 jours, tandis que le nombre de bactéries sporulées était de 3.81±1.21×106/g. Nous n'avons trouvé ni bactéries anaérobies ni bactéries coliformes fécales dans aucun des échantillons.Bacillus cereus, Staphylococcus epidermis, B. licheniformis, Streptococcus faecalis, Serratia liquefaciens etAspergillus niger furent les micro-organismes le plus souvent isolés. Nous n'avons pas trouvé de bactéries ou de champignons pathogénes primaires, mais la présence de pathogènes opportunistes indiquait qu'une surveillance assidue de l'équipement de production du virus et un contrôle rigoureux de la qualité des lots de production sont nécessaires.
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Podgwaite, J.D., Bruen, R.B. & Shapiro, M. Microorganisms associated with production lots of the nucleopolyhedrosis virus of the gypsy moth,Lymantria dispar [Lep.: Lymantriidae] . Entomophaga 28, 9–15 (1983). https://doi.org/10.1007/BF02372092
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DOI: https://doi.org/10.1007/BF02372092