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Nitric oxide modulates pancreatic edema formation in rat caerulein-induced pancreatitis

  • Liver, Pancreas, and Biliary Tract
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Abstract

This study was designed to investigate the role of nitric oxide (NO) in the formation of pancreatic edema in caerulein-induced pancreatitis in rats. Pancreatitis was produced by two intraperitoneal injections of caerulein, and plasma amylase concentration, pancreatic edema index (pancreatic wet weight/body weight), and Evans blue extravasation (as a measure of vascular permeability) were evaluated 5h after the first injection. Four doses (1, 2.5, 5, and 10 mg/kg) of NG-nitro-L-arginine (l-NNA), an NO synthase inhibitor, were subcutaneously administered at −0.5, 0.5, 1.5, 2.5, and 3.5h after the first injection of caerulein.l-NNA significantly lowered the edema index, the wet/dry weight ratio of the pancreas, and Evans blue extravasation in the rats with pancreatitis. The maximal effect was obtained byl-NNA at a dose of 2.5 mg/kg; this inhibited the increase in pancreatic edema formation from the control value by 60%–70%. Intraperitoneal injections (20 mg/kg, five times) ofl-arginine, a substrate for NO production, partly reversed thel-NNA-induced inhibition of pancreatic edema formation, butd-arginine, an enantiomer ofl-arginine, did not show any effect. Plasma amylase concentrations were not significantly affected by any dose of L-NNA, nor were they affected byl- ord-arginine. These findings strongly suggest that endogenous NO plays an important role in the formation of pancreatic edema in caerulein-induced pancreatitis in rats, probably by increasing vascular permeability and protein extravasation.

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Authors and Affiliations

  1. The Third Department of Internal Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, 980, Sendai, Miyagi, Japan

    Takashi Abe, Tooru Shimosegawa, Akihiko Satoh, Reishi Abe, Yoshifumi Kikuchi, Masaru Koizumi & Takayoshi Toyota

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  1. Takashi Abe
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  2. Tooru Shimosegawa
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  3. Akihiko Satoh
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  4. Reishi Abe
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  5. Yoshifumi Kikuchi
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  6. Masaru Koizumi
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  7. Takayoshi Toyota
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Abe, T., Shimosegawa, T., Satoh, A. et al. Nitric oxide modulates pancreatic edema formation in rat caerulein-induced pancreatitis. J Gastroenterol 30, 636–642 (1995). https://doi.org/10.1007/BF02367791

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  • DOI: https://doi.org/10.1007/BF02367791

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