Summary
When using two restriction enzymes and DNA probes from different sources, high levels of restriction fragment length polymorphism (RFLP) were observed in Southern blot hybridisation experiments, among 6 di(ha)ploid genotypes ofSolanum tuberosum and some closely related species. In 3 F1 sample populations of 14 individuals each, both heterozygosity and segregation of hybridisation patterns were observed. The non-radioactive biotin-dUTP/SA-AP method for DNA detection on Southern blots proved to be satisfactory and reliable.
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Jacobs, J.J.M.R., Krens, F.A., Stiekema, W.J. et al. Restriction fragment length polymorphism inSolanum spp for the construction of a genetic map ofSolanum tuberosum L.: a preliminary study. Potato Res 33, 171–180 (1990). https://doi.org/10.1007/BF02358444
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DOI: https://doi.org/10.1007/BF02358444