Summary
A cuprozinc superoxide dismutase has been isolated from pig liver. The enzyme is similar to previously described cuprozinc superoxide dismutases in that it is a dimer of about 32 000 molecular weight consisting of approximately two equally sized subunits, and 2 atoms of copper and two atoms of zinc per molecule. It differs, however, from previously described cuprozinc superoxide dismutases because of its higher isoelectric point; pI 6.8 vs 4.9 for bovine enzyme. The diffusion coefficient for the porcine enzyme was determined to be 7.53×10−7 cm2s−1, while the equivalent spherical hydrodynamic radius was computed as 28.5 Å. The enzyme was observed to undergo self-association with time. Sulfhydryl interaction is postulated to be involved.
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Salin, M.L., Wilson, W.W. Porcine superoxide dismutase. Mol Cell Biochem 36, 157–161 (1981). https://doi.org/10.1007/BF02357032
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DOI: https://doi.org/10.1007/BF02357032