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Androgen receptor in rat Harderian and submandibular glands

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Summary

Androgens regulate the development and sexual dimorphism of rodent Harderian and submandibular glands. This effect is believed to be mediated by the androgen receptor. Immunohistochemistry and immunoblotting were carried out to study the receptor in normal, castrated and dihydrotestosterone-supplemented rat Harderian and submandibular glands. Immunohistochemically, the most intense nuclear staining was observed in the acinar cells of the submandibular glands, followed by intercalated duct cells. The granular convoluted tubules showed weak immunostaining and the striated ducts were negative. In the Harderian gland, nuclear staining was seen in both type I and II secretory cells. Castration and treatment had no effect on the expression of the androgen receptor protein in either gland. A 110 K androgen receptor signal was detected by immunoblotting in the Harderian gland but not in the submandibular gland. An experiment was designed to explore the possible effect of proteinases on the receptor protein in the homogenate of submandibular gland. Our results demonstrate the cell-specific location of the receptor in Harderian and submandibular glands, and show that the expression of the receptor protein is androgen-independent.

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Authors and Affiliations

  1. Molecular Endocrinology Research Unit and Graduate School of Steroid Research, Department of Anatomy, Tampere University Medical School, FIN-33101, Tampere, Finland

    Ya-Hua Zhuang, Merja Bläuer & Pentti Tuohimaa

  2. Institute of Medical Technology, University of Tampere, Finland

    Heimo Syvälä

  3. Institute of Dentistry, University of Turku, Finland

    Merja Laine

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  1. Ya-Hua Zhuang
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  2. Merja Bläuer
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  3. Heimo Syvälä
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  4. Merja Laine
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  5. Pentti Tuohimaa
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Zhuang, YH., Bläuer, M., Syvälä, H. et al. Androgen receptor in rat Harderian and submandibular glands. Histochem J 28, 477–483 (1996). https://doi.org/10.1007/BF02331407

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  • DOI: https://doi.org/10.1007/BF02331407

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