Synopsis
The purpose of the present investigation was to determine at what stages of embryonic development the sodium pump (Na+,K+-activated ATP phosphohydrolase, EC 3.6.1.3) appears and whether the ontogeny of the sodium pump plays a role in the matrotrophic viviparity ofSebastes. Early larval stages (stages 1–14) had embryonic tissues nearly devoid of Na, K-ATPase activity. After epiboly, tissues from embryos taken from both species,S. schlegeli andS. taczanowskii, had significant levels of enzymatic activity coincident with the appearance of neuronal tissue in the head fold (stages 15–20). Maximal levels of specific activities of the enzyme were reached with the onset of the vascular circulation and the opening of the buccal cavity together with maturation of the midgut and hindgut regions of the intestinal tract (stages 26 and 28). Ouabain, a specific inhibitor of Na, K-ATPase, was used to measure survival of embryos placed in different seawater concentrations under in vitro conditions. The most sensitive stages to external ouabain were 26–30. These findings support the hypothesis that the intestinal tract is functional prior to gestation and could be transporting important nutritive material found in maternal ovarian fluid.
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Conte, F.P., Takano, K., Takemura, A. et al. Ontogeny of the sodium pump in embryos of rockfish of the genusSebastes . Environ Biol Fish 30, 127–134 (1991). https://doi.org/10.1007/BF02296883
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DOI: https://doi.org/10.1007/BF02296883