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Rapid detection ofCampylobacter jejuni andCampylobacter coli isolated from clinical specimens using the polymerase chain reaction

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Abstract

SeventeenCampylobacter strains isolated from 16 children hospitalised with acute diarrhea were analysed by in vitro enzymatic amplification using two sets of oligonucleotide primers specific forCampylobacter jejuni andCampylobacter coli, respectively. Thirteen strains (76 %) were identified asCampylobacter jejuni and four strains (24 %) asCampylobacter coli. Subsequent bacteriological identification confirmed the identity of the same 13Campylobacter jejuni strains and the 4Campylobacter coli strains. Thus, these PCR methods enabled rapid and specific detection of all theCampylobacter jejuni andCampylobacter coli strains without any false-positive or false-negative results.

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Authors and Affiliations

  1. Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, 25 rue du Dr. Roux, 75724, Paris Cedex 15, France

    V. Stonnet & J. L. Guesdon

  2. Laboratory of Microbiology, University N. Testemitanu, 165 bd. Stefan cel Mare, 277017, Chisinau, Republic of Moldova

    L. Sicinschi

  3. Laboratoire de Bactériologie-Enfants, Hôpital Pellegrin, 33076, Bordeaux, France

    F. Mégraud

Authors
  1. V. Stonnet
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  2. L. Sicinschi
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  3. F. Mégraud
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  4. J. L. Guesdon
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Stonnet, V., Sicinschi, L., Mégraud, F. et al. Rapid detection ofCampylobacter jejuni andCampylobacter coli isolated from clinical specimens using the polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis. 14, 355–359 (1995). https://doi.org/10.1007/BF02116533

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  • DOI: https://doi.org/10.1007/BF02116533

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