Abstract
Properties of isocitrate lyase fromEscherichia coli, the first enzyme of the glyoxylate bypass, have been compared from cells grown on either acetate or glycolate as the sole carbon source. Michaelis constants for isocitrate, isoelectric points, native and subunit molecular weights, antigenic properties, peptide mapping with V-8 or trypsin, and several other properties were examined. Our data suggest that only one isocitrate lyase form exists inE. coli regardless of carbon source used for growth.
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Zarembinski, C.M., Hoyt, J.C. & Reeves, H.C. Properties of isocitrate lyase fromEscherichia coli K12 grown on acetate or glycolate. Current Microbiology 22, 65–68 (1991). https://doi.org/10.1007/BF02106215
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DOI: https://doi.org/10.1007/BF02106215