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A new member of a secretory protein gene family in the dipteranChironomus tentans has a variant repeat structure

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Summary

We describe the structure of a gene expressed in the salivary gland cells of the dipteranChironomus tentans and show that it encodes 1 of the approximately 15 secretory proteins exported by the gland cells. This sp115,140 gene consists of approximately 65 copies of a 42-bp sequence in a central uninterrupted core block, surrounded by short nonrepetitive regions. The repeats within the gene are highly similar to each other, but divergent repeats are present in a pattern which suggests that the repeat structure has been remodeled during evolution. The 42-bp repeat in the gene is a simple variant of the more complex repeat unit present in the Balbiani ring genes, encoding four of the other secretory proteins. The structure of the sp115,140 gene suggests that related repeat structures have evolved from a common origin and resulted in the set of genes whose secretory proteins interact in the assembly of the secreted protein fibers.

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Author notes

  1. Christer Ericsson

    Present address: Molecular Biology and Virology Laboratory, The Salk Institute, PO Box 85800, 92138, San Diego, California, USA

  2. Mats Carlquist

    Present address: Karo Bio, Box 4032, 141 04, Huddinge, Sweden

Authors and Affiliations

  1. Department of Molecular Genetics, Medical Nobel Institute, Karolinska Institutet, Box 60400, S-104 01, Stockholm, Sweden

    Joakim Galli, Urban Lendahl, Gabrielle Paulsson, Christer Ericsson & Lars Wieslander

  2. Department of Physiological Chemistry, Karolinska Institutet, Box 60400, S-104 01, Stockholm, Sweden

    Tomas Bergman

  3. Department of Biochemistry II, Karolinska Institutet, Box 60400, S-104 01, Stockholm, Sweden

    Mats Carlquist

Authors
  1. Joakim Galli
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  2. Urban Lendahl
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  4. Christer Ericsson
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  7. Lars Wieslander
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Galli, J., Lendahl, U., Paulsson, G. et al. A new member of a secretory protein gene family in the dipteranChironomus tentans has a variant repeat structure. J Mol Evol 31, 40–50 (1990). https://doi.org/10.1007/BF02101791

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  • DOI: https://doi.org/10.1007/BF02101791

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