Summary
We have sequenced the coding region for the small-subunit rRNA gene fromParamecium tetraurelia. Similarity comparisons between smallsubunit rRNAs from representative of the Metazoa, the Plantae, the Fungi and four other members of the Ciliophora were used to construct phylogenetic trees. In these phylogenies the Ciliophora diverged from the eukaryotic line of descent as a loose phylogenetic grouping during a radiative period that gave rise to the Fungi, the Plantae and the Metazoa.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brosius J, Palmer ML, Kennedy PJ, Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene fromEscherichia coli. Proc Natl Acad Sci USA 75:4801–4805
Chan Y, Gutell R, Noller HF, Wool IG (1984) The nucleotide sequence of a rat 18S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18S ribosomal ribonucleic acid. J Biol Chem 259:224–230
Corliss JO (1979) The ciliated Protozoa: characterization, classification, and guide to literature, 2nd edn. Pergamon Press, New York, p 455
Elwood HJ, Olsen GJ, Sogin ML (1985) The small subunit ribosomal RNA gene sequences from the hypotrichous ciliatesOxytricha nova andStylonychia pustulata. Mol Biol Evol 2:399–410
Fitch WM, Margoliash E (1967) Construction of phylogenetic trees: A method based on mutation distances as estimated from cytochrome C sequences is of general applicability. Science 155:279–284
Fox GE, Stackebrandt E, Hespell RB, Gibson J, Maniloff J, Dyer TA, Wolfe RS, Balch WE, Tanner RS, Magrum LJ, Zablen LB, Blakemore R, Gupta R, Bonen L, Lewis BJ, Stahl DA, Luehrsen KR, Chen KN, Woese CR (1980) The phylogeny of prokaryotes. Science 209:457–463
Gupta R, Lanter JM, Woese CR (1983) Sequence of the 16S ribosomal RNA fromHalobacterium volcanii, an archaebacterium. Science 221:656–659
Hori H, Osawa S (1979) Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species. Proc Natl Acad Sci USA 76:381–385
Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic Press, New York, pp 21–132
Maniatis T, Fritsch EF, Sambrook J (1982) Large-scale isolation of plasmid DNA. In: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 86–94
Mankin AS, Kopylov AM, Bogdanov AA (1981) Modification of 18S rRNA in the 40S ribosomal subunit of yeast with dimethylsulfate. FEBS Lett 134:11–14
McCarroll R, Olsen GJ, Stahl YD, Woese CR, Sogin ML (1983) Nucleotide sequence ofDictyostelium discoideum small-subunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications. Biochemistry 22:5858–5868
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Messing J, Carlson J, Hagen G, Rubenstein I, Oleson A (1984) Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region. DNA 3:31–40
Noller HF, Woese CR (1981) Secondary structure of 16S ribosomal RNA. Science 212:403–411
Olsen GJ, McCarroll R, Sogin ML (1983) Secondary structure of theDictyostelium discoideum small subunit ribosomal RNA. Nucleic Acids Res 11:8037–8049
Ozaki T,Koshikawa Y, Iida Y, Iwabuchi M (1984) Sequence analysis of the transcribed and 5′ non-transcribed regions of the ribosomal RNA gene inDictyostelium discoideum.Nucleic Acids Res 12:4171–4184
Panet A, van de Sande JH, Loewen PC, Khorana HG, Raae AJ, Lillehaug JR, Kleppe K (1973) Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry 12:5045–5050
Rubtsov PM, Musakhanov MM, Zakharyev VM, Krayev AS, Skryabin KG, Bayev AA (1980) The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal gene fromSaccharomyces cerevisiae. Nucleic Acids Res 8:5779–5794
Salim M, Maden BEH (1981) Nucleotide sequence ofXenopus laevis 18S ribosomal RNA inferred from gene sequence. Nature 291:205–208
Sanger F, Coulson AR (1975) A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol 94:441–448
Small EB, Lynn DH (1981) A new macrosystem for the phylum Ciliophora Doflein, 1901. Biosystems 14:387–401
Sogin ML, Swanton MT, Gunderson JH, Elwood HJ (1986) Sequence of the small subunit ribosomal RNA gene from the hypotrichous ciliateEuplotes aediculatus. J Protozool 33:26–29
Spangler EA, Blackburn EH (1985) The nucleotide sequence of the 17S ribosomal RNA gene ofTetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromycin and hygromycin. J Biol Chem 160:6334–6340
Stackebrandt E, Woese CR (1981) Evolution of prokaryotes. In: Carlite MJ, Collins JF, Moseley BEB (eds) Molecular and vellular aspects of microbial evolution. Cambridge University Press, Cambridge, England, pp 1–31
Takaiwa F, Oono K, Sugiura M (1984) The complete nucleotide sequence of a rice 17S rRNA gene. Nucleic Acids Res 12: 5441–5448
Vogelstein B, Gillespie D (1979) Preparative and analytic purification of DNA from agarose. Proc Natl Acad Sci USA 76: 615–619
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sogin, M.L., Elwood, H.J. Primary structure of theParamecium tetraurelia small-subunit rRNA coding region: Phylogenetic relationships within the caliophora. J Mol Evol 23, 53–60 (1986). https://doi.org/10.1007/BF02100998
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02100998