Abstract
A restriction map of the plasmid pMA1 ofMicrocystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB 5-2-4. A transcript of 1800 b was detected. The minicell-producing strain X 984 ofEscherichia coli was transformed with pMA1 cloned into pUC18. Two polypeptides sized 50 kD and 43 kD respectively were synthesized in a specific manner in transformed cells; this indicates that the cyanobacterial transcription and translation signals are recognized byE. coli.
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Schwabe, W., Hübschmann, T., Meixner, M. et al. Transcription and in vivo expression of aMicrocystis aeruginosa plasmid. Current Microbiology 20, 365–368 (1990). https://doi.org/10.1007/BF02095861
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DOI: https://doi.org/10.1007/BF02095861