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Proliferating cell nuclear antigen (PCNA) expression in regenerating rat liver after partial hepatectomy

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Abstract

Proliferating cell nuclear antigen (PCNA) is a nuclear protein maximally elevated in the S phase of proliferating and transformed cells and is recognized by the monoclonal antibody PC-10 in paraffin tissue sections. The liver regenerative process after partial hepatectomy in rats was estimated with thein vivo incorporation of [3H]thymidine into liver DNA and the liver thymidine kinase activity. The expression of PCNA in rat liver after partial hepatectomy was performed by immunohistochemical staining with PC-10 in paraffin embedded tissues, at different time intervals up to 240 hr. Proliferating cell nuclear antigen expression, [3H]thymidine incorporation into DNA, and liver thymidine kinase activity exhibited marked oscillations during the liver regenerative process. A close relationship was demonstrated among DNA synthesis, thymidine kinase activity, and PC-10 score. Our results suggest that PC-10 monoclonal antibody may be used as a worthwhile proliferation index in the evaluation of the rate of liver regeneration in rats.

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Authors and Affiliations

  1. From the Department of Experimental Pharmacology, Medical School, University of Athens, 115 27 Goudi, Athens, Greece

    Stamatios E. Theocharis MD, Antigone S. Skopelitou MD, PhD, Alexandra P. Margeli PhD, Kitty J. Pavlaki MD, PhD & Christos Kittas MD, PhD

  2. Pathology Department, Medical School, University of Athens, 115 27 Goudi, Athens, Greece

    Stamatios E. Theocharis MD, Antigone S. Skopelitou MD, PhD, Alexandra P. Margeli PhD, Kitty J. Pavlaki MD, PhD & Christos Kittas MD, PhD

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  1. Stamatios E. Theocharis MD
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  2. Antigone S. Skopelitou MD, PhD
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  3. Alexandra P. Margeli PhD
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  4. Kitty J. Pavlaki MD, PhD
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Theocharis, S.E., Skopelitou, A.S., Margeli, A.P. et al. Proliferating cell nuclear antigen (PCNA) expression in regenerating rat liver after partial hepatectomy. Digest Dis Sci 39, 245–252 (1994). https://doi.org/10.1007/BF02090193

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  • DOI: https://doi.org/10.1007/BF02090193

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