Abstract
The relatively complex growth-factor requirements of two strains ofSphaerotilus discophorus, strains 31 and 32, have been elucidated. In addition to thiamin and biotin which are required by other strains ofS. discophorus, the two strains must be supplied also with adenine or guanine and with cyanocobalamin for growth in a glucose — Casamino Acids — mineral salts medium. The cyanocobalamin can be replaced by methionine but only if relatively large amounts of this amino acid, 100 µg or more per ml, are added to the medium. The methionine requirement of the two strains is approximately 3 times greater than that of otherS. discophorus strains.
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Stokes, J.L., Johnson, A.H. Growth factor requirements of two strains ofSphaerotilus discophorus . Antonie van Leeuwenhoek 31, 175–180 (1965). https://doi.org/10.1007/BF02045888
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DOI: https://doi.org/10.1007/BF02045888