Abstract
In a previous project on haematoporphyrin (HP) uptake by experimentally induced cholesteatoma of the rabbit, it was shown that HP concentration was remarkably high in the cholesteatoma, with a peak at three hours after intravenous administration of the drug. On the basis of these results an attempt was made to destroy the matrix of cholesteatoma by means of photodynamic therapy.
Cholesteatomas were induced bilaterally in the ears of eight rabbits which were divided into two groups, according to the interval between HP administration (5 mg/kg body weight) and irradiation. The irradiation was carried out with light at 600–680 nm, filtered from the emission a quartz halogen lamp (dose rate 300 mW cm−2; total dose 120 J cm−2). The first group was submitted to photodynamic therapy after three hours and the second after twelve hours. In both groups half the rabbits were sacrificed at 24 h and half at 48 h post-irradiation.
Histological studies showed significant vascular lesions, necrosis of the wall of the cholesteatoma, interstitial extravasation of blood, and oedema in a high percentage of the animals. The lesions were much greater in animals sacrificed at 48 h after photodynamic therapy.
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References
Dougherty TJ, Boyle DG, Weishaupt K et al. Phototherapy of human tumors. In: Castellani A (ed)Research in Photobiology. New York: Plenum 1977:435
Auler H, Banger C. Untersuchungen Uber die Rolle der Porphyrine bei geschwulstrkranken Menschen und Tieren.Z Krebsforsch 1942,53:65–8
Beltramini F, Ricchelli G, Jori G et al. Interaction of hematoporphyrin with isolated lipoprotein fractions: a spectroscopic study. In: Jori G, Perria C. (eds)Photodynamic Therapy of Tumors and other Diseases. Padova: Edizioni Libreria Progetto 1985:81
Jori G. Utilization of liposomes and low density lipoproteins as porphyrin carriers in experimental photodynamic therapy. Proc. of the First Internat. Conf. on the Clinical Applications of Photosensitization for Diagnosis and Treatment. Tokyo, Japan 1986:83
Teatini GP, Perria C, Jori G et al. Experimental study on hematoporphyrin uptake by cholesteatoma.Arch Otorhinolaryngol 1988:in press
Zulch KJ.Histology of Brain Tumors. Berlin: Springer-Verlag 1971:250 (Fig. 97 (a) and (b))
Steinbach E. Tierexperimentelle Untersuchungen zue Erzeugung von Cholesteatomen.Laryng Rhinol 1978,57:724–33
Berenbaum MC, Hall GW, Hoyes, AD. Cerebral photo-sensitization by hematoporphyrin derivative: Evidence for an endothelial site of action.Br J Cancer 1986,53:81–9
Star WM, Marijnissen JPA, van den Berg-Blok AB et al. Destructive effect of photoradiation on the microcirculation of a rat mammary tumour growing in ‘sandwich’ observation chambers. In: Doiron DR, Gomer CJ (eds)Porphyrin Localization and Treatment of Tumors. New York: Alan R Liss, 1984:637
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Perria, C., Teatini, G., Tanda, F. et al. Photodynamic treatment of cholesteatoma of the ear induced experimentally in rabbits: Preliminary report. Laser Med Sci 4, 111–113 (1989). https://doi.org/10.1007/BF02032607
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DOI: https://doi.org/10.1007/BF02032607