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Neutrophil migration induced by inflammatory stimuli is reduced by macrophage depletion

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Abstract

Previous experiments of our group have shown that neutrophil migration induced by inflammatory stimuli is reduced by agents which block the release from macrophages of a specific factor for neutrophil migration (MNCF, [1, 2]). The present paper evaluated the influence of macrophage depletion induced by lavage of the peritoneal cavity on neutrophil migration. In both normal and thioglycollatestimuled peritoneal cavities, lavage with saline reduced the resident macrophage population by about 80% and significantly blocked neutrophil migration induced by inflammatory stimuli such as carrageenin, zymosan andE. coli endotoxin. Peritoneal lavage, however, did not affect neutrophil migration induced by MNCF. Thus, these results support the suggestion that macrophages participate in the control of neutrophil migration induced by acute inflammatory stimuli.

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References

  1. F. Q. Cunha and S. H. Ferreira,The release of a neutrophil chemotactic factor from peritoneal macrophages by endotoxin: inhibition by glucocorticoids. European J. Pharmacol.129, 65–76 (1986).

    Article  Google Scholar 

  2. G. E. P. Souza and S. H. Ferreira, Blockade by anti-macrophage serum of the migration of PMN neutrophils into the inflamed peritoneal cavity. Agents and Actions17, 97–103 (1985).

    Article  PubMed  Google Scholar 

  3. J. A. Kazmierowski, J. I. Gallin and H. Y. Reynolds,Mechanism for the inflammatory response in primate lungs. Demonstration and partial characterization of an alveolar macrophage-derived factor with preferential activity for polymorphonuclear leukocytes. J. Clin. Invest.59, 273–281 (1977).

    PubMed  Google Scholar 

  4. G. W. Hunninghake, J. I. Gallin and A. S. Fauci,Immunologic reactivity of the lung. The in vivo and in vitro generation of a neutrophil chemotactic factor by alveolar macrophages. Am. Rev. Resp. Dis.117, 15–23 (1978).

    PubMed  Google Scholar 

  5. I. Y. R. Adamson and D. H. Bowden,Chemotactic and mitogenic components of the alveolar macrophages response to particles and neutrophil chemoattractants. Am. J. Path.109, 71–77 (1982).

    PubMed  Google Scholar 

  6. W. Merril, G. P. Naegel, R. A. Matthay and H. Y. Reynolds,Production of chemotactic factor(s) by in vitro cultur ed human alveolar macrophages. Clin. Res.26, 635a (1978).

    Google Scholar 

  7. S. H. Ferreira, G. E. P. de Souza and R. Ribeiro dos Santos,In vivo inhibition of PMN migration by antimacrophage serum. In:Inflammation: Mechanisms and Treatment. (Ed. D. A. Willoughby and J. P. Giroud) pp. 789–793. MTP Press Limited, Lancaster 1980.

    Google Scholar 

  8. R. Vinegar, J. F. Truax, J. L. Selph and F. A. Voelker,Pathway of onset, development and decay of carrageenin pleurisy in the rat. Federation Proc.41, 2588–2595 (1982).

    Google Scholar 

  9. W. W. Merril, G. P. Naegel, R. A. Matthay and H. Y. Reynolds,Alveolar macrophages-derived chemotactic factor. Kinetics of in vitro production and partial characterization. J. Clin. Invest.65, 268–276 (1980).

    PubMed  Google Scholar 

  10. H. Y. Reynolds,Lung inflammation: role of endogenous chemotactic factors in attracting polymorphonuclear granulocytes. Am. Rev. Resp. Dis.127, 516–525 (1983).

    Google Scholar 

  11. F. Q. Cunha, G. E. P. Souza and S. H. Ferreira,Macrophages stimulated with lipopolysaccharide release a selective neutrophil chemotactic factor: An “in vivo” demonstration. Brazilian J. Med. Biol. Res.19, 775–777 (1986).

    Google Scholar 

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Authors and Affiliations

  1. Departments of Pharmacology of the Faculty of Pharmaceutical Sciences and of the Faculty of Medicine of Ribeirão Preto, University of São Paulo, 14049, Ribeirão Preto, SP, Brazil

    G. E. P. Souza, F. Q. Cunha, R. Mello &  S. H. Ferreira

  2. Section of Pharmacology and Toxicology-INCQS-Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil

    G. E. P. Souza, F. Q. Cunha, R. Mello &  S. H. Ferreira

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  1. G. E. P. Souza
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  2. F. Q. Cunha
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  3. R. Mello
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  4. S. H. Ferreira
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Souza, G.E.P., Cunha, F.Q., Mello, R. et al. Neutrophil migration induced by inflammatory stimuli is reduced by macrophage depletion. Agents and Actions 24, 377–380 (1988). https://doi.org/10.1007/BF02028296

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  • DOI: https://doi.org/10.1007/BF02028296

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