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Use of commercial particle agglutination systems for the rapid identification of methicillin-susceptible and methicillin-resistantStaphylococcus aureus

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Abstract

The performance of a recently introducedStaphylococcus aureus identification system (Slidex Staph-Kit) was compared with that of currently available systems (Immuno Scan Staphlatex, Staphyloslide, Staphaurex and SeroSTAT II) for the identification of methicillin-resistantStaphylococcus aureus (MRSA) and methicillin-susceptibleStaphylococcus aureus. The new system, which detects a capsular antigen common in MRSA, performed with equal or greater sensitivity than the other systems. None of the commercial systems was adversely affected by the methicillin susceptibility of the staphylococci when isolates were recovered from non-selective media. The greatest advantage of the anti-capsular monoclonal reagent was its improved performance on isolates recovered from selective media.

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References

  1. Kloos WE, Lambe DW Staphylococcus. In: Balows A, Hausler WJ, Herrmann KL, Isenberg HD, Shadomy HJ (ed): Manual of clinical microbiology. American Society for Microbiology, Washington DC, 1991, p. 222–237.

    Google Scholar 

  2. Piper J, Hadfield T, McCleskey F, Evans M, Friedstrom S, Lauderdale P, Winn R Efficacies of rapid agglutination tests for identification of methicillin-resistant staphylococcal strains asStaphylococcus aureus. Journal of Clinical Microbiology 1988, 26: 1907–1909.

    PubMed  Google Scholar 

  3. Wanger AR, Morris SL, Ericsson C, Singh KV, LaRocco MT Latex agglutination negative methicillin-resistantStaphylococcus aureus recovered from neonates: epidemiologic features and comparison of typing methods. Journal of Clinical Microbiology 1992, 30: 2583–2588.

    PubMed  Google Scholar 

  4. Ruane PJ, Morgan MA, Citron DM, Mulligan ME Failure of rapid agglutination methods to detect oxacillin-resistantStaphylococcus aureus. Journal of Clinical Microbiology 1986, 24: 490–492.

    PubMed  Google Scholar 

  5. Woolfrey BF, Lally RT, Ederer MN An evaluation of three rapid coagglutination tests: SeroSTAT, AccuStaph, and Staphloslide, for differentiatingStaphylococcus aureus from other species of staphylococci. American Journal of Clinical Pathology 1984, 81: 345–348.

    PubMed  Google Scholar 

  6. Fournier M, Boutonnier A, Bouvet A Staphylococcus aureus strains which are not identified by rapid agglutination methods are of capsular serotype 5. Journal of Clinical Microbiology 1989, 27: 1372–1374.

    PubMed  Google Scholar 

  7. Fournier JM, Bouvet A, Boutonnier A, Audurier A, Goldstein F, Pierre J, Bure A, Lebrun L, Hochkeppel HK Predominance of capsular type 5 among oxacillin-resistantStaphylococcus aureus. Journal of Clinical Microbiology 1987, 25: 1932–1933.

    PubMed  Google Scholar 

  8. Fournier JM, Bouvet A, Mathieu D, Nato F, Boutonnier A, Gerbal R, Brunengo P, Saulnier C, Sagot N, Slizewicz B, Mazie JC New latex reagent using monoclonal antibodies to capsular polysaccharide for reliable identification of both oxacillin-susceptible and oxacillin-resistantStaphylococcus aureus. Journal of Clinical Microbiology 1993, 31: 1342–1344.

    PubMed  Google Scholar 

  9. Van Enk RA, Thompson KD Use of a primary isolation medium for recovery of methicillin-resistantStaphylococcus aureus. Journal of Clinical Microbiology 1992, 30: 504–505.

    PubMed  Google Scholar 

  10. National Committee for Clinical Laboratory Standards Performance standards for antimicrobial disk susceptibility tests. Fourth edition. Approved standard M2-A4. NCCLS, Villanova, PA, 1990.

    Google Scholar 

  11. Lairscey R, Buck GE Performance of four slide agglutination methods for identification ofStaphylococcus aureus when testing methicillin-resistant staphylococci. Journal of Clinical Microbiology 1987, 25: 181–182.

    PubMed  Google Scholar 

  12. Smith SM, Berezny C Comparative evaluation of identification systems for testing methicillin-resistant strains ofStaphylococcus aureus. Journal of Clinical Microbiology 1986, 24: 173–176.

    PubMed  Google Scholar 

  13. Berke A, Tilton RC Evaluation of rapid coagulase methods for the identification ofStaphylococcus aureus. Journal of Clinical Microbiology 1986, 23: 916–919.

    PubMed  Google Scholar 

  14. Punsalang, A, Migneault PC, Nolte FS Reliability of latex agglutination tests for identification ofStaphylococcus aureus resistant to oxacillin. Journal of Clinical Microbiology 1986, 24: 1104–1106.

    PubMed  Google Scholar 

  15. Branger C, Goullet P, Boutonnier A, Fournier JM Correlation between esterase electrophoretic types and capsular polysaccharide types 5 and 8 among methicillin-susceptible and methicillin-resistant strains ofStaphylococcus aureus. Journal of Clinical Microbiology 1990, 28: 150–151.

    PubMed  Google Scholar 

  16. Naidu AS, Paulsson M, Wadstrom T Particle agglutination assays for rapid detection of fibronectin, fibrinogen, and collagen receptors onStaphylococcus aureus. Journal of Clinical Microbiology 1988, 26: 1549–1554.

    PubMed  Google Scholar 

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  1. Pathology and Laboratory Medicine Service (113), Veterans Administration Medical Center, 4100 West Third Street, 45428, Dayton, Ohio, USA

    J. Adams & R. Van Enk

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  1. J. Adams
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  2. R. Van Enk
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Adams, J., Van Enk, R. Use of commercial particle agglutination systems for the rapid identification of methicillin-susceptible and methicillin-resistantStaphylococcus aureus . Eur. J. Clin. Microbiol. Infect. Dis. 13, 86–89 (1994). https://doi.org/10.1007/BF02026132

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