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Characterization of an adenylate cyclase system sensitive to histamine H2-receptor excitation in cells from dog gastric mucosa

  • Histamine and Kinins
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Abstract

A method of preparing viable cells from dog gastric mucosa is described. Cyclic AMP in these cells is elevated by histamine and 4-methyl histamine but 2-methyl histamine is only a weak agonist. The effects on cyclic AMP levels are inhibited competitively by metiamide and burimamide which give apparent KBvalues of 3.5×10−7 M and 2.3×10−6 M, respectively. These values are similar to those reported for other histamine H2-receptor systems. The H1-receptor antagonists, mepyramine and chlorpheniramine, have no inhibitory effect on the histamine induced elevation of cyclic AMP: promethazine inhibits the system but not by a competitive mechanism. It is concluded that the histamine stimulated adenylate cyclase system is probably located in the parietal cell component.

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Authors and Affiliations

  1. Department of Biochemistry, Imperial Chemical Industries Limited, Pharmaceuticals Division, Mereside, Alderley Park, SK 10 4TG, Macclesfield, Cheshire

    P. Scholes, Ann Cooper, D. Jones, J. Major, M. Walters & C. Wilde

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  1. P. Scholes
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  2. Ann Cooper
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  3. D. Jones
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  4. J. Major
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  5. M. Walters
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Scholes, P., Cooper, A., Jones, D. et al. Characterization of an adenylate cyclase system sensitive to histamine H2-receptor excitation in cells from dog gastric mucosa. Agents and Actions 6, 677–682 (1976). https://doi.org/10.1007/BF02026087

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  • DOI: https://doi.org/10.1007/BF02026087

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