Summary
Several heavy metals have direct calcifying effects in connective tissues; most notable among them is lead (Pb), whether administered topically (1) or systemically with local injury (2). The mechanisms of metal-induced soft tissue calcification have been studied by injecting salts of known in vitro calcifying potential into subcutaneous connective tissue (3); of the metals used, only lead and holmium produced an early accumulation of minerals on collagen in vivo. Lead is also claimed to accelerate bone healing in the rabbit leg with no toxic effects (4).
Lead-loaded ion-exchange resin beads, implanted into surgically prepared subcutaneous pouches in rats, rapidly induced subcutaneous calcification which has been studied by microradiography and electron-probe microanalysis.
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MacDonald, N.S., Ezmirlian, F., Spain, McArthur, C.: The ultimate site of skeletal deposition of strontium and lead, J. Biol. Chem.189:387–399, 1951
Selye, H., Gabbiani, G., Tuchweber, B.: Factors influencing topical calcinosis induced by trauma following intravenous injection of lead acetate, Arch. Int. Pharmacodyn. Ther.145:254–263, 1963
Gabbiani, G., Tuchweber, B., Perrault, G.: Studies in the mechanism of metal-induced soft tissue calcification, Calcif. Tissue Res.6:20–31, 1970
Bethge, J.F.J., Koch, G., Kohrt, T. Ch., Schlosser, G.-A., Schmieding, I.: Experimental acceleration of fracture healing, Langenbecks Arch. Klin. Chir.328:371–381, 1971
Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy, J. Ultrastruct. Res.26:31–43, 1969
Malcolm, A.S.: A lapping apparatus for hard tissue sections, Aust. Dent. J.20:32–36, 1975
Fleish, H., Neuman, W.F.: Mechanisms of calcification: role of collagen, polyphosphates, and phosphatase, Am. J. Physiol.200:1296–1300, 1961
Schiffmann, E., Corcoran, B.A., Martin, G.R.: The role of complexed heavy metals in initiating the mineralization of “Elastin” and the precipitation of mineral from solution, Arch. Biochem.115:87–94, 1966
Cousins, F.B., Smillie, A.C.: Studies on a skin calcifying system, Aust. J. Exp. Biol. Med. Sci.43:785–802, 1965
Verzár, F., Freydberg-Lucas, V.: Calcium45 uptake and turnover of tendon fibres as influenced by thermic contraction and age, Gerontologia2:11–21, 1958
Verzár, F., Huber, K.: The affinity of collagen fibres for radioisotopes Ca45, Na24, K42 and I131 during thermic contraction, Gerontologia2:113–121, 1958
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Ellender, G., Sewell, D.K.B., Ham, K.N. et al. Lead-loaded ion-exchange resin bead as a calcergen. Calc. Tis Res. 26, 253–258 (1978). https://doi.org/10.1007/BF02013267
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DOI: https://doi.org/10.1007/BF02013267