Skip to main content
SpringerLink
Log in

Characterization of potassium channels in mast cell plasma membranes: Their possible existence and involvement in potassium-evoked histamine release

  • Plenary Lecture
  • Histamine Release
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

The effects of three K+-channel blockers, quinine, quinidine and sparteine on K+-evoked histamine (HA) release have been investigated. KCl (150 mM) initiated 40–55% release of HA from rat peritoneal mast cells in the absence of extracellular Ca++, and this could be suppressed by pretreating the cells with K+-channel blockers. In the concentration range of 0.05–2 mM, reductions were dose-dependent and the IC50-s (in mM) obtained were 0.15 and 0.25 for quinine and quinidine and 0.20 for sparteine. The light microscopic studies showed close parallelism between the KCl-evoked HA-release and mast cell degranulation in the absence and also in the presence of K+-channel blockers. Present results strenghthened the involvement of K+-channels in the KCl-evoked HA release and showed that these channels are sensitive to quinine/quinidine and also to sparteine. These data appeared, however, insufficient to clarify fully the mechanism of K+-induced HA secretion or to classify the K+-channels involved in this mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. Németh, P. Magyar, R. Herceg and Z. Huszti,Potassium-induced release of histamine from mast cells and its inhibition by ketotifen. Agents and Actions20, 149–152, 1987.

    PubMed  Google Scholar 

  2. A. Németh, P. Magyar and Z. Huszti,Inhibition of potassium-induced release of histamine from mast cells by tetraethylammonium and tetramethylammonium. Agents and Actions30, 143–145, 1990.

    Article  PubMed  Google Scholar 

  3. O. P. Hamil,Membrane ion channels: (in Burgen, Roberts, eds) Topics in molecular pharmacology, Elsevier, Amsterdam, 1983, pp. 182–205.

    Google Scholar 

  4. I. Findlay, M. Dunne, S. Ulrich, C. B. Wollheim and O. H. Petersen,Quinine inhibits Ca ++-independent K +-channels whereas tetraethylammonium inhibits Ca ++-activated K +-channels in insulin-secreting cells. FEBS Letters185, 4–7, 1985.

    Article  PubMed  Google Scholar 

  5. A. L. Kleinhaus,Segregation of leech neurones by the effect of sparteine on action potential duration. J. Physiol.299, 309–321, 1980.

    PubMed  Google Scholar 

  6. P. Shore, A. Burkhalter and V. H. Cohn,A method for fluorimetric assay of histamine in tissues. J. Pharm. exp. Ther.127, 182–186, 1959.

    Google Scholar 

  7. G. Paolisso, M. Nenquin, W. Schmeer, F. Mathot, H. P. Meissner and C. Henquin,Sparteine increases insulin release by decreasing the K +-permeability of the B-cell membrane. Biochem. Pharmacol.34, 2355–2361, 1985.

    Article  PubMed  Google Scholar 

  8. E. A. Newman,Distribution of potassium conductance in mammalian Muller (glial) cells: a comparative study. J. Neurosci.7, 2423–2432, 1987.

    PubMed  Google Scholar 

  9. M. Armando-Hardy, J. C. Ellory, H. G. Ferreira, S. Fleminger,Inhibition of calcium-induced increase in the potassium permeability of human red blood cells by quinine, Proc. Phys. Soc.250, 32–33P, 1975.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy II, Semmelweis University of Medicine, Budapest, Hungary

    A. Németh

  2. Department of Pharmacodynamics, Semmelweis University of Medicine, Budapest, Hungary

    Z. Huszti

Authors
  1. A. Németh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. Huszti
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Németh, A., Huszti, Z. Characterization of potassium channels in mast cell plasma membranes: Their possible existence and involvement in potassium-evoked histamine release. Agents and Actions 36 (Suppl 2), C308–C310 (1992). https://doi.org/10.1007/BF01997359

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01997359

Keywords

Search

Navigation