Skip to main content
SpringerLink
Log in

Colony formation ofDrosophila cells in semisolid medium containing agarose

  • Short Communications
  • Cellular Biology
  • Published:
Experientia Aims and scope Submit manuscript

Summary

FourD. melanogaster established cell lines were tested for their ability to form clones. A plating efficiency of about 0.5% was achieved with a medium containing agarose, reaching 1% with agarose supplemented with a small amount of conditioned medium. Each clone, examined cytologically, displayed a specific and stable karyotype.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Dolfini, S., Chromosoma33 (1971) 196.

    Article  CAS  PubMed  Google Scholar 

  2. Mosna, G. and Dolfini, S., Chromosoma38 (1972) 1.

    Article  CAS  PubMed  Google Scholar 

  3. Mosna, G., Rc. Ist., lomb. Sci. Lett.113 (1979) 17.

    Google Scholar 

  4. Halfer, C., Privitera, E. and Barigozzi, C., Chromosoma76 (1980) 201.

    Article  Google Scholar 

  5. Echalier, G., Curr. Topics Microbiol. Immun.55 (1971) 220.

    CAS  Google Scholar 

  6. Bernhard, H.P. and Gehring, W.J., Experientia31 (1975) 734.

    Google Scholar 

  7. Nakajima, S. and Miyake, T., in: Invertebrate Tissue Culture. Applications in Medicine, Biology and Agriculture, p. 279 Eds. E. Kurstak and K. Maramorosch. Academic Press, New York 1976.

    Google Scholar 

  8. Richard-Molard, C., and Ohanessian, A., Wilhelm Roux Arch.181 (1977) 135.

    Article  Google Scholar 

  9. Wyss, C., Somatic Cell Genet.5 (1979) 23.

    Article  CAS  PubMed  Google Scholar 

  10. Schneider, I., and Blumenthal, B., in: The Genetics and Biology of Drosophila, vol. 2a, p. 265. Eds. M. Ashburner and T.R.W. Wright, Academic Press, New York 1978.

    Google Scholar 

  11. Mosna, G., and Barigozzi, C., Experientia32 (1976) 855.

    Article  CAS  PubMed  Google Scholar 

  12. Echalier, G., and Ohanessian, A., In Vitro6 (1970) 162.

    Article  CAS  PubMed  Google Scholar 

  13. Hayflick, L., in: Tissue Culture; Methods and Applications, p. 722. Eds. P.F. Kruse Jr and M.K. Patterson Jr. Academic Press, New York 1973.

    Chapter  Google Scholar 

  14. Fogh, J., and Fogh, H., Proc. Soc. exp. Biol. Med.117 (1964) 899.

    Article  CAS  PubMed  Google Scholar 

  15. Macpherson, I., in: Tissue Culture; Methods and Applications, p. 276. Eds. P.F. Kruse Jr and M.K. Patterson Jr. Academic Press, New York 1973.

    Chapter  Google Scholar 

  16. Macpherson, I., and Russell, W., Nature210 (1966) 1343.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Biologia, Sezione di Genetica, Via Celoria 26, I-20133, Milano, Italy

    G. Mosna

Authors
  1. G. Mosna
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The author whishes to thank Prof. A. Ghidoni for critical reading of the manuscript and Mrs A. Bonifazio for her technical assistance.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mosna, G. Colony formation ofDrosophila cells in semisolid medium containing agarose. Experientia 39, 774–775 (1983). https://doi.org/10.1007/BF01990321

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01990321

Keywords

Search

Navigation