Abstract
The neutrophil chemotaxins, complement fragment C5a (C5a) and GROα, induced the mobilization of Ca2+ from intracellular stores and the polymerization of actin in human neutrophils as assayed by flow cytometric measurements. [Ca2+]i-transients developed as an “all-or-none” response. Individual neutrophils required different threshold concentrations of added ligand to induce [Ca2+]i-transients which were then always maximal. In contrast, chemotaxin-induced formation of actin filaments in single neutrophils occurred in a dose-dependent manner. Pertussis toxin blocked chemotaxin-induced actin polymerization and [Ca2+]i-transients indicating that both cell responses shared initial activation steps such as ligand binding and activation of guanine nucleotide-binding proteins (G-proteins).
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Abbreviations
- [Ca2+]i :
-
Cytosolic free Ca2+
- C5a:
-
Complement fragment C5a
- PtdlnsP2 :
-
Phosphatidylinositol (4,5)-bisphosphate
- IP3 :
-
Inositol-trisphosphate
- fluo-3:
-
fluo-3-acethoxymethyl ester
- NBD-phallacidin:
-
7-nitrobenz-2-oxa-1,3-diazol-phallacidin
- EGTA:
-
[(2-(aminoethyl-glycolether-N,N,N′,N′-tetraacidic acid]
- f-actin:
-
filament actin
- PT:
-
pertussis toxin
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Metzner, B., Elsner, J., Dobos, G. et al. [Ca2+]i-transients and actin polymerization in human neutrophils under stimulation with GROα and complement fragment C5a. Agents and Actions 42, 101–106 (1994). https://doi.org/10.1007/BF01983473
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DOI: https://doi.org/10.1007/BF01983473