Abstract
The influence of serotonin (5-HT) probably released in reactions of delayed-type hypersensitivity (DTH) on the locomotion of lymphocytes was investigated. 5-HT has a chemokinetic and a chemotactic action on the movement of enriched T-lymphocytes. This 5-HT activity is inhibited by 5-HT antagonists as 2-bromolysergic acid diethylamide, methysergide and pizotiphen. The metabolic product of 5-HT the 5-hydroxyindoleacetic acid has the same activity as 5-HT itself, whereas 5-hydroxytryptophan is without significant influence on the lymphocyte migration. Thus it is supposed that the action of 5-HT on the lymphocyte movement is a specific effect. Lymphocytes are also chemokinetically stimulated by the mediators of the DTH, the lymphokines. 5-HT and lymphokines given together lead to an additive effect. The physiological importance of the described results have to be evidenced forin vivo conditions.
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References
L.V. Devoino andR. Yu. Ilyutchenok,Influence of some drugs on the immune response. II. Effects of serotonin, 5-hydroxytryptophan, reserpine and iproniacid on delayed hypersensitivity, Eur. J. Pharmac.4, 449–456 (1968).
R.K. Gershon, P.W. Askenase andM.D. Gershon,Requirement for vasoactive amines for production of delayed-type hypersensitivity skin reactions, J. exp. Med.142, 732–747 (1975).
A. Schwartz, P. W. Askenase andR.K. Gershon,The effect of locally injected vasoactive amines on the elicitation of delayed-type hypersensitivity, J. Immun.118, 159–165 (1977).
M. Hagen andI. Paegelow,5-Hydroxytryptamine-releasing activity in culture supernatants of guinea-pig lymphocytes, Agents and Actions, in press.
P.W. Askenase, S. Bursztajn, M.D. Gershon andR.K. Gershon,T cell-dependent mast cell degranulation and release of serotonin in murine delayed-type hypersensitivity, J. exp. Med.152, 1358–1374 (1980).
I. Paegelow andP. Lange, Pharmacological studies on lymphocytes. 1. Effect of 5-hydroxytryptamine, bradykinin and lymphokines on the migration of lymphocytes in vitro. InTrends in Inflammation Research, vol. 2, pp. 255–265 (EdsH. Bekemeier, R. Hirschelmann). Birkhäuser, Basel 1982.
M.H. Julius, E. Simpson andL.A. Herzenberg,A rapid method for the isolation of functional thymusderived murine lymphocytes, Eur. J. Immun.13, 645–649 (1973).
E.G. Maderazo andC.l. Woronick,Micropore filter assay of human granulocyte locomotion: problems and solutions, Clin. Immun. Immunopath.11, 196–211 (1978).
S.H. Zigmond andJ.G. Hirsch,Leukocyte locomotion and chemotaxis. New methods for evaluation and demonstration of a cell-derived chemotactic factor, J. exp. Med.137, 387–410 (1973).
N.D. Tsakalos, T.C. Theoharides, S. Kraeuter Kops, J.M. Dwyer andP.W. Askenase,Dialyzable leukocyte extract induces mast cell secretion, Cell. Immun.66, 383–393 (1982).
S. Garattini andL. Valzelli,Serotonin, pp. 265–271. Elsevier Publishing Company, Amsterdam, London, New York 1965.
D.S. Dumonde, The rheumatological significance of lymphokines. InThe recognition of Anti-Rheumatic Drugs, 167–291 (Eds.D.C. Dumonde andU.K. Tasani). MTP Press, Lancaster, England 1978.
D. Shepro andP.A. D'Amore,Endothelial cell metabolism, Adv. Microcirculat.9, 161–205 (1980).
P.A. Ward, Ch.D. Offen andJ.R. Montgomery,Chemoattractants of leukocytes with special references to lymphocytes, Fedn Proc.30, 1721–1724 (1971).
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Paegelow, I., Lange, P. Pharmacological studies on lymphocytes: 2. Effects of indole derivatives and their antagonists on the lymphocyte migration. Agents and Actions 15, 361–366 (1984). https://doi.org/10.1007/BF01972372
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DOI: https://doi.org/10.1007/BF01972372