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Methods for determining ploidy in amphibians: Nucleolar number and erythrocyte size

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Summary

Diploid and triploidXenopus can be easily and reliably distinguished by the size of their erythrocytes. This method has several advantages over other methods, such as counting metaphase chromosomes and counting nucleoli. One problem with the latter method is the reduction in cells with a full complement of nucleoli when regenerating tissue is used.

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Author notes

  1. Michelle R. Lennartz

    Present address: Dept. of Biochemistry, University of Michigan, 48104, Ann Arbor, MI, USA

Authors and Affiliations

  1. Biology Department and Neuroscience Program, Amherst College, 01002, Amherst, MA, USA

    S. A. George & Michelle R. Lennartz

Authors
  1. S. A. George
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  2. Michelle R. Lennartz
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Additional information

Research supported by NIH grant EY 01662.

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George, S.A., Lennartz, M.R. Methods for determining ploidy in amphibians: Nucleolar number and erythrocyte size. Experientia 36, 687–688 (1980). https://doi.org/10.1007/BF01970141

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  • DOI: https://doi.org/10.1007/BF01970141

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