Summary
A microbial sensor system was prepared for the detemination of methane. This microbial sensor consisted of immobilizedMethylomonas sp. and an oxygen electrode. When sample gas containing methane was applied to the system the current in the electrode decreased with time until a steady-state was reached. The steady-state current was attained within 3 min and the maximum drop in current was obtained at 30 °C and pH 7.2. The time required for the determination of methane was 1 min. A linear relationship was observed between the current difference and the methane gas concentration, below 6.6 mM. The minimum gas concentration required for the determination of methane was 13.1 μM. The current difference was reproducible within 5%. The current output of the sensor was almost constant for more than 20 days and 500 assays.
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Okada, T., Karube, I. & Suzuki, S. Microbial sensor system which usesMethylomonas sp. for the determination of methane. European J. Appl. Microbiol. Biotechnol. 12, 102–106 (1981). https://doi.org/10.1007/BF01970042
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DOI: https://doi.org/10.1007/BF01970042