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Anticomplementary fraction from the poisonous secretion of the paratoid gland of the toad (Bufo marinus paracnemis Lutz)

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Summary

Fractionation of the poisonous secretion of the toadBufo marinus paracnemis Lutz, by dialysis and chromatography on QAE-Sephadex, led to the isolation of a fraction which was adsorbed to the ion exchanger. This fraction, when incubated with human serum, yielded an anticomplementary effect that was evaluated by measuring the kinetics of lytic activity on sensitized sheep red cells (classical pathway) and unsensitized rabbit cells (alternative pathway).

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Authors and Affiliations

  1. Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences, USP, 14.100, Ribeirão Preto, SP, Brazil

    A. I. de Assis, J. E. Barbosa & I. F. de Carvalho

  2. Department of Parasitology, Microbiology and Immunology, and Department of Medicine, Faculty of Medicine, USP, 14.100, Ribeirão Preto, SP, Brazil

    A. I. de Assis, J. E. Barbosa & I. F. de Carvalho

Authors
  1. A. I. de Assis
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  2. J. E. Barbosa
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  3. I. F. de Carvalho
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Additional information

We are very much indebted to Dr Lewis J. Greene for valuable discussions. Thanks are due to Dr Antonio C. Nero and Miss Ester G. Silva for their cooperation. This work was supported in part by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).

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de Assis, A.I., Barbosa, J.E. & de Carvalho, I.F. Anticomplementary fraction from the poisonous secretion of the paratoid gland of the toad (Bufo marinus paracnemis Lutz). Experientia 41, 940–942 (1985). https://doi.org/10.1007/BF01970023

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  • DOI: https://doi.org/10.1007/BF01970023

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