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Dietary effects of pristane on rat lymphoid tissues

  • Inflammation and Immunomodulation
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Abstract

Studies were conducted to assess the normal tissue-associated levels of pristane (2,6,10,14-tetramethyl-pentadecane) in Copenhagen rats during ontogeny and adult life and to address whether or not dietary pristane can be adsorbed from the gut and disseminated throughout the body. During the course of this study the possible effects of dietary pristane on chromatin conformation of lymphoid cells were also examined by flow cytometry. The data indicated that 1) pristane crossed the placenta and accumulated in fetal tissues, 2) neonates were exposed to pristane via the colostrum, 3) there were significant increases in the amount of tissue-associated pristane in young adults and subsequent redistribution of the pristane to the muscle and adipose tissues in older rats and 4) after dietary exposure, significantly elevated levels of pristane were associated with the tissues and concomitant changes in chromatin conformation were observed. Collectively, these results suggest that pristane was adsorbed from dietary sources, disseminated to the tissues and exerted a transient, yet marked effect on chromatin of lymphoid cells in rats.

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Abbreviations

PI:

propidium iodide

3-MC:

3-methylcholanthrene

TPA:

12-O-tetradecanoylphorbol 13-acetate

PP:

Peyer's patches

MLN:

mesenteric lymph nodes

ALN:

axillary lymph nodes

PBS:

phosphate buffered saline

GLC:

gas-liquid chromatography

References

  1. R. A. Dean and E. V. Whitehead,The occurrence of phytane in petroleum. Tetrahedron Lett.21, 768–770 (1961).

    Google Scholar 

  2. J. G. Bendoraitis, B. L. Brown and L. S. Hepner,Isolation and identification of isoprenoids in petroleum. InProceedings of the 6th World Petroleum Congress, Section V, 1963.

  3. J. Oro' and D. W. Nooner,Hydrocarbons in lipid extracts of algal mats. Fed. Proc.25, 768 (1966).

    Google Scholar 

  4. M. Blumer, M. M. Mullin and D. W. Thomas,Pristane in zooplankton. Science140, 974 (1963).

    Google Scholar 

  5. B. Hallgren and S. Larsson,Separation of pristane from herring. Acta Chem. Scand.17 543–545 (1963).

    Google Scholar 

  6. N. A. Sorensen and J. Mehlum,Studies related to pristane. I. The unsaponifiable matter of the liver oil of the basking shark. Acta Chem. Scand.2, 140–146 (1948).

    Google Scholar 

  7. P. N. Anderson and M. Potter,Induction of plasma cell tumors in BALB/c mice with 2,6,10,14-tetramethylpentadecane (pristane) Nature222, 994–995 (1969).

    PubMed  Google Scholar 

  8. F. C. S. Ho and K. H. Fu,A new model of AA-amyloidosis induced by oral pristane in BALB/c mice. Br. J. Exp. Pathol.68, 413–420 (1987).

    PubMed  Google Scholar 

  9. B. R. Brodeur, P. Tsang and Y. Larose,Parameters affecting ascites tumour formation in mice and monoclonal antibody production. J. Immuno. Methods71, 265–272 (1984).

    Google Scholar 

  10. N. Warner,Autoimmunity and the pathogenesis of plasma cell tumor induction in NZB inbred and hybrid mice. Immunogenetics2, 1–20 (1975).

    Google Scholar 

  11. M. Cancro and M. Potter,The requirement of an adherent cell substratum for the growth of developing plasmacytoma cells in vivo. J. Exp. Med.144, 1554–1567 (1976).

    PubMed  Google Scholar 

  12. M. L. Kripke and D. W. Weiss,Studies on the immune responses of BALB/c mice during tumor induction by mineral oil. Int. J. Cancer6, 422–430 (1970).

    PubMed  Google Scholar 

  13. K. L. Bost, L. R. Garrett and M. A. Cuchens,The role of the Peyer's patches in carcinogenesis. II. 3-methylcholanthrene induced lymphoid malignancies in rat Peyer's patches. Carcinogenesis7, 1257–1265 (1986).

    PubMed  Google Scholar 

  14. L. R. Garrett, K. L. Bost, T. M. Buttke and M. A. Cuchens,Changes in the DNA of lymphocytes from pristane treated rats. Agents and Actions20, 104–112 (1987).

    PubMed  Google Scholar 

  15. L. R. Garrett, D. W. Pascual, L. W. Clem and M. A. Cuchens,Conformational changes in the DNA of hybridoma cells from pristane treated mice. Chem.-Biol. Interactions61, 249–263 (1987).

    Google Scholar 

  16. J. Avigan, G. W. A. Milne and R. J. Highet,The occurrence of pristane and phytane in man and animals. Biochim. Biophys. Acta144, 127–131 (1967).

    PubMed  Google Scholar 

  17. H. J. O'Neill, L. L. Gershbein and R. G. Scholz,Identification of pristane in human sebum and related lipid sources. Biochem. Biophys. Res. Commun.35, 946–952 (1959).

    Google Scholar 

  18. A. Boyum,Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest.21, (suppl. 97), 1–30 (1968).

    Google Scholar 

  19. E. G. Bligh and W. J. Dyer,A rapid method of total lipid extraction and purification. Can. J. Biochem. Phys.37, 911–917 (1959).

    Google Scholar 

  20. A. M. Le Bon, J. P. Cravedi and J. E. Tulliez,Disposition and metabolism of pristane in rat. Lipids23, 424–429 (1988).

    PubMed  Google Scholar 

  21. F. F. Becker and T. J. Slaga,Critical Molecular Determinants of Carcinogenesis, vol. 39. The Univ. of Texas M. D. Anderson Symp. on Fund. Cancer Research, Univ. of Texas Press, Austin 1987.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, 39216-4505, Jackson, MS, USA

    L. R. Garrett, J. -G. Chung, P. E. Byers & M. A. Cuchens

Authors
  1. L. R. Garrett
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  2. J. -G. Chung
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  3. P. E. Byers
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  4. M. A. Cuchens
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Additional information

This investigation was supported by PHS grant CA 33111-04.

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Garrett, L.R., Chung, J.G., Byers, P.E. et al. Dietary effects of pristane on rat lymphoid tissues. Agents and Actions 28, 272–278 (1989). https://doi.org/10.1007/BF01967414

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  • DOI: https://doi.org/10.1007/BF01967414

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