Summary
Few C6 glioma cells synthesize the astroglia-specific GFA protein in monolayer culture. A uniform population of GFA-positive cells was obtained by aggregating C6 cells in suspension culture, as previously reported for C6 glioma maintained on sponge foam matrices. These results strongly suggest that cell-to-cell interactions promote GFA expression.
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Acknowledgments. This work was supported by USPHS grant No. 13034 and by the Veterans Administration.
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Bignami, A., Swanson, J. & Dahl, D. GFA expression in aggregating cultures of rat C6 glioma. Experientia 35, 1170–1171 (1979). https://doi.org/10.1007/BF01963267
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DOI: https://doi.org/10.1007/BF01963267