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Comparative evaluation of 7 helminth antigens in the enzyme-linked immunosorbent assay (E.L.I.S.A.)

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Summary

112 sera from Europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (E.L.I.S.A.) against 6 crude extracts of various helminths (2 of adult worms:Dipetalonema viteae, Fasciola hepatica; 3 of eggs:Ascaris suum, Toxocara canis, Schistosoma mansoni; and ofEchinococcus granulosus scolices) and against bovine hydatid fluid. Each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. Extensive cross-reactions were observed, leading to the conclusion that non-purified helminth antigens, even in combination, are of limited value for reliable serodiagnosis in E.L.I.S.A.

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Authors and Affiliations

  1. Department of Medicine, Swiss Tropical Institute, Socinstrasse 57, CH-4051, Basel, (Switzerland)

    F. Speiser & N. Weiss

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  1. F. Speiser
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  2. N. Weiss
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Speiser, F., Weiss, N. Comparative evaluation of 7 helminth antigens in the enzyme-linked immunosorbent assay (E.L.I.S.A.). Experientia 35, 1512–1514 (1979). https://doi.org/10.1007/BF01962817

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  • DOI: https://doi.org/10.1007/BF01962817

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