Abstract
The performance of the MB-Check system (Roche) in the recovery of mycobacteria from clinical specimens was compared with that of the Bactec radiometric system and conventional culture on Löwenstein-Jensen medium. A total of 1,582 clinical specimens were used for the study; 74 yielded mycobacteria. Organisms of theMycobacterium tuberculosis andMycobacterium avium complexes accounted for the majority of isolates. The rate of recovery of all mycobacteria with the Bactec system was 91.9%, with the MB-Check system 79.7% and on Löwenstein-Jensen medium only 54.1%. Combination of the Bactec and MB-Check systems allowed recovery of 100% of isolates. The Bactec system showed a faster detection time than the other two methods.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Morgan MA, Horstmeier CD, DeYoung DR, Roberts GD: Comparison of a radiometric method (Bactec) and conventional culture media for the recovery of mycobacteria from smear-negative specimens. Journal of Clinical Microbiology 1983, 18: 384–388.
Laszlo A, Siddigui SH: Evaluation of a rapid radiometric differentiation test for theMycobacterium tuberculosis complex by selective inhibition with pnitro-acetyl-amino β-hydroxypropiophenone. Journal of Clinical Microbiology 1984, 19: 694–698.
Fadda G, Roe SL: Recovery and susceptibility testing ofMycobacterium tuberculosis from extrapulmonary specimens by the Bactec radiometric method. Journal of Clinical Microbiology 1984, 19: 720–721.
Park CH, Hixon DL, Ferguson CB, Hall SL, Risheim CC, Cook CB: Rapid recovery of mycobacteria from clinical specimens using an automated radiometric technique. American Journal of Clinical Pathology 1984, 81: 341–345.
Kirihara JM, Hillier SL, Coyle MB: Improved detection times forMycobacterium avium complex andMycobacterium tuberculosis with Bactec radiometric system. Journal of Clinical Microbiology 1985, 22: 841–845.
Anargyros P, Astill DSJ, Lim ISL: Comparison of improved Bactec and Löwenstein-Jensen media for culture of mycobacteria from clinical specimens. Journal of Clinical Microbiology 1990, 28: 1288–1291.
Stager CE, Libonati JP, Siddigui SH, Davis JR, Hooper NM, Baker JF, Carter ME: Role of solid media when used in conjunction with the Bactec system for mycobacterial isolation and identification. Journal of Clinical Microbiology 1991, 29: 154–157.
Vestal AL: Procedure for isolation and identification of mycobacteria. DHEW Publication no. 7682301. Centers for Disease Control, Atlanta, 1975, p. 97–115.
Roberts GD, Koneman EW, Kim JK: Mycobacterium. In: Balows A (ed): Manual of clinical microbiology. American Society for Microbiology, Washington, DC, 1991, p. 304–339.
Giger T, Burkardt HJ: Evaluation of a new biphasic system for the recovery of mycobacteria. European Journal of Clinical Microbiology & Infectious Diseases 1990, 9: 428–431.
Isenberg HD, D'Amato RF, Heifets L, Murray PR, Scardamaglia M, Jacobs MC, Alperstein P, Niles A: Collaborative feasibility study of a biphasic system (Roche Septi-Check AFB) for rapid detection and isolation of mycobacteria. Journal of Clinical Microbiology 1991, 29: 1719–1722.
Abe C, Hosojima S, Fukasawa Y, Kazumi Y, Takahashi M, Hirano K, Mori T: Comparison of MB-Check, Bactec and egg-based media for recovery of mycobacteria. Journal of Clinical Microbiology 1992, 30: 878–881.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Piersimoni, C., Morbiducci, V., De Sio, G. et al. Comparative evaluation of the MB-check system for recovery of mycobacteria from clinical specimens. Eur. J. Clin. Microbiol. Infect. Dis. 11, 1174–1177 (1992). https://doi.org/10.1007/BF01961140
Issue Date:
DOI: https://doi.org/10.1007/BF01961140