Summary
We have found that Zn2+ prevented lysis of unfertilized sea urchin eggs, and the eggs retained the ability to form fertilization membranes and to divide. SDS polyacrylamide gel electrophoresis showed that proteolysis of several proteins accompanied egg lysis, but Zn2+ inhibited this proteolysis. Therefore, Zn2+ blocks protease activity directly or indirectly and thereby prolongs the longevity of unfertilized sea urchin eggs.
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References
Harvey, E. B., The American Arbacia and Other Sea Urchins, Princeton University Press, New Jersey 1956.
Hinegardner, R., in: The Sea Urchin Embryo: Biochemistry and Morphogenesis, p. 10–25. Ed. G. Czihak. Springer-Verlag Berlin/Heidelberg/New York 1975.
Aketa, K., Zool. Sci,1 (1984) 918.
Aketa, K., Zool. Sci,2 (1985) 943.
Boobis, A. R., Fawthrop, D. J., and Davies, D.S., Trends pharmac. Sci.10 (1989) 275.
Parchment, R. E., and Pierce, G. B., Cancer Res.49 (1989) 6680.
Bradford, M. M., Analyt. Biochem.72 (1976) 248.
Laemmli, U. K., Nature (London)227 (1970) 680.
Morrissey, J. H., Analyt. Biochem.117 (1981) 307.
Lallier, R., in: The Sea Urchin Embryo: Biochemistry and Morphogenesis, p. 473–509. Ed. G. Czihak. Springer-Verlag, Berlin/Heidelberg/New York 1975.
Kobayashi, N., Publ. Seto mar. Biol. Lab.18 (1971) 379.
Murakami, H., Hayakawa, M., Fujii, T., Hara, T., Itami, Y., Kishida, A., and Nishida, I., Okayama Igakkai Zasshi88 (1976) 39 (in Japanese).
Nakamura, S., Ohmi, C., and Kojima, M. K., Zool. Sci.6 (1989) 329.
Sugiyama, M., Zool. Mag. (Tokyo)58 (1949) 137 (in Japanese).
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Nakamura, S., Oda, N., Nakamura, K. et al. Zinc increases the longevity of unfertilized sea urchin eggs. Experientia 47, 734–736 (1991). https://doi.org/10.1007/BF01958830
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DOI: https://doi.org/10.1007/BF01958830