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Heterologous mischarging as a means of tRNA fractionation. II. Isolation of E. coli tRNA1Val and tRNA1Ala

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Summary

Highly purified E. coli tRNA1 Val and tRNA1 Ala have been isolated, based on the properties of heteroaminoacylated tRNAs and their behaviour on BD-cellulose chromatography.

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Authors and Affiliations

  1. Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile

    Gloria León, N. Ruiz, L. Meza & M. Krauskopf

Authors
  1. Gloria León
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  2. N. Ruiz
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  3. L. Meza
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  4. M. Krauskopf
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Additional information

Acknowledgments. We thank Prof. H. Peña for his help in the preparation of the manuscript. This work was supported in part by the Research Fund of the Universidad Austral de Chile.

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León, G., Ruiz, N., Meza, L. et al. Heterologous mischarging as a means of tRNA fractionation. II. Isolation of E. coli tRNA1Val and tRNA1Ala. Experientia 33, 1029–1030 (1977). https://doi.org/10.1007/BF01945950

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  • DOI: https://doi.org/10.1007/BF01945950

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