Skip to main content
SpringerLink
Log in

Patterns of membrane organization in toad bladder epithelium: A freeze-fracture study

  • Specialia
  • Anatomica, Histologica, Embryologica, Carcinologica
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Two cell-types of toad bladder epithelium show uncommon plasma membrane organization in freeze-fractured specimens. One type, the granular cell, contains a plasma membrane in which the A-face is poorly particulate luminally while the B-face discloses multiple large particles at this site. In contrast, the lateral and basal portions of the granular-cell membrane are typical in that more particles occupy the A-face than the B-face. In the other cell-type, which is mitochondriarich, the plasma membrane, luminally, laterally, and basally, contains rod-shaped and a few glubular particles in the A-face. We suggest that these two peculiar membrane organizations by considered in the localization of both vasopressin and aldosterone action in toad bladder.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. M. Hays andS. D. Levine, Kidney Int.6, 307 (1974).

    Article  CAS  PubMed  Google Scholar 

  2. A. Leaf, Ergebn. Physiol.56, 215 (1965).

    Article  Google Scholar 

  3. J. H. Ludens andD. D. Fanestil, Am. J. Physiol.223, 1338 (1972).

    Article  CAS  PubMed  Google Scholar 

  4. F. Humbert, C. Pricam, A. Perrelet andL. Orci, Eur. J. clin. Invest.4, 344 (1974).

    Google Scholar 

  5. D. Branton, Phil. Trans. R. Soc. Lond.261, 133 (1971).

    CAS  Google Scholar 

  6. F. Bastide andS. Jard, Biochim. biophys. Acta150, 113 (1968).

    Article  CAS  PubMed  Google Scholar 

  7. H. Moor, K. Mühlethaler, H. Waldner andA. Frey-Wyssling, J. biochem. biophys. Cytol.10, 1 (1961).

    Article  CAS  Google Scholar 

  8. J. K. Choi, J. Cell Biol.16, 53 (1963).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. D. R. DiBona, M. M. Civan andA. Leaf, J. Membrane Biol.1, 79 (1969).

    Article  CAS  Google Scholar 

  10. D. R. DiBona, M. M. Civan andA. Leaf, J. Cell Biol.40, 1 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. J. B. Wade, M. J. Karnovsky andV. A. DiScala, J. Cell Biol.63, 360a (1974).

    Google Scholar 

  12. J. Chevalier, J. Bourguet andJ. S. Hugon, Cell Tiss. Res.152, 129 (1974).

    Article  CAS  Google Scholar 

  13. D. Danon, J. M. Strum andI. S. Edelman, J. Membrane Biol.16, 279 (1974).

    Article  CAS  Google Scholar 

  14. W. L. Davis, D. B. P. Goodman, J. H. Martin andJ. L. Matthews, J. Cell Biol.61, 544 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. F. Spinelli, A. Grosse andR. C. de Sousa, J. Membrane Biol.,23, 139 (1975).

    Article  CAS  Google Scholar 

  16. M. M. Civan andH. S. Frazier, J. gen. Physiol.51, 589 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. A. D. C. Macknight, A. Leaf andM. M. Civan, Biochim. biophys. Acta222, 560 (1970).

    Article  CAS  PubMed  Google Scholar 

  18. W. N. Scott andV. S. Sapirstein, Science184, 797 (1974).

    Article  CAS  PubMed  Google Scholar 

  19. S. K. Masur, E. Holtzman andR. Walter, J. Cell Biol.52, 211 (1972).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. L. Orci, A. Grosso andR. C. de Sousa, Experientia30, 707 (1974).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Histology and Embryology, and Department of Physiology and Medicine, University of Geneva, School of Medicine, CH-1211, Genève 4, Switzerland

    L. Orci, Fabienne Humbert, Mylène Amherdt, Alda Grosso, R. C. de Sousa & A. Perrelet

Authors
  1. L. Orci
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fabienne Humbert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mylène Amherdt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alda Grosso
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. C. de Sousa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Perrelet
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Some of these results were presented in abstract form at the 6th annual meeting of the Swiss Societies for Experimental Biology, Lausanne, 11–12, May 197421

The authors are indebted to Mrs.Marthe Sidler-Ansermet, Mrs.Gorana Perrelet, MissMariella Ravazzola and Mr.Michel Bernard for valuable assistance.

This work was supported in part by grants No. 3.0311.73, 3.8081.72, 3.553.75 and 3.1300.73 from Fonds National Suisse de la Recherche Scientifique, Bern, Switzerland, and by a grant-in-aid from Hoechst-Pharmaceuticals (Frankfurt-Hoechst, W. Germany).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orci, L., Humbert, F., Amherdt, M. et al. Patterns of membrane organization in toad bladder epithelium: A freeze-fracture study. Experientia 31, 1335–1338 (1975). https://doi.org/10.1007/BF01945814

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01945814

Keywords

Search

Navigation