Summary
The level of some enzymatic activities in red blood cells before and after photohemolysis induced by protoporphyrin IX was studied. A 30% decrease in catalase activity was found both in normal erythrocytes and those from patients affected by favism. Other proteins though present in larger amounts inside the erythrocytes are not affected by the photohemolytic process.
Similar content being viewed by others
References
E. S. Peterka, W. J. Runge and R. M. Fusaro, Archs Derm.94, 282 (1966).
B. D. Goldstein and L. C. Harber, J. clin. Invest.51, 892 (1972).
A. A. Lamola, T. Yamane and A. M. Trozzolo, Science179, 1131 (1973).
A. F. P. M. De Goeij, J. Th. Ververgaert and J. Van Steveninck, Clin. chim. Acta62, 287 (1975).
A. W. Girotti, Biochem. biophys. Res. Commun.72, 1367 (1976).
R. Strom, C. Crifò, S. Mari, G. Federici, I. Mavelli and A. Finazzi-Agrò Physiol. chem. Phys.,9, 63 (1977).
H. Lück, in: Methods of Enzymatic analysis, 2nd ed., p. 886. Ed. M. V. Bergmeyer. Verlag Chemie/Academic Press, New York 1974.
H. P. Misra and I. Fridovich, J. biol. Chem.247, 3170 (1972).
V. Pocker and J. T. Stone, Biochemistry6, 668 (1967).
E. D. Paglia and W. N. Valentine, J. Lab. Med.70, 158 (1967).
G. L. Ellman and E. Callaway, Nature,192, 1216 (1961).
J. W. Harris and R. W. Kellermayer, in: The red Cell, p. 559. Harward Univ. Press, Harward 1970.
A. Finazzi-Agrò, D. Cocco, L. Calabrese, W. H. Bannister and F. Bossa, Int. J. Biochem., in press (1977).
A. Bozzi, I. Mavelli, A. Finazzi-Agrò, R. Strom, A. M. Wolf, B. Mondovi and G. Rotilio, Molec. Cell. Biol.10, 11 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Finazzi-Agrò, A., Fadda, M.B., Floris, G. et al. Selective inactivation of catalase during protoporphyrin induced photohemolysis of human red blood cells. Experientia 34, 181–182 (1978). https://doi.org/10.1007/BF01944663
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01944663