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Transfer of isolated nuclei into protoplasts of Aspergillus nidulans

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Abstract

Nuclei were isolated from protoplasts of a haploid auxotrophicAspergillus nidulans strain. Transformation of protoplasts prepared from a complementary haploid auxotrophic strain with these purified nuclei resulted in both heterokaryotic and diploid colonies. The nutritionally-complementing colonies appeared at a frequency of 5×10−7 to 10−8.

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Authors and Affiliations

  1. Department of Microbiology, Attila József University, P.O. Box 533, H-6701, Szeged, Hungary

    Cs. Vágvölgyi & L. Ferenczy

Authors
  1. Cs. Vágvölgyi
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  2. L. Ferenczy
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Vágvölgyi, C., Ferenczy, L. Transfer of isolated nuclei into protoplasts of Aspergillus nidulans. Experientia 48, 271–272 (1992). https://doi.org/10.1007/BF01930473

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  • DOI: https://doi.org/10.1007/BF01930473

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