Abstract
The tritium-labeled bis-norleucine analog ofHelicoverpa zea pheromone biosynthesis-activating neuropeptide ([3H]NLPBAN) was incubated in vitro with hemolymph fromManduca sexta orH. zea adult females. The incubations resulted in the formation of several tritium-labeled degradation products. At a [3H]NLPBAN concentration of 0.9 μM the degradation proceeded at a very slow but physiologically plausible rate (2–10 fmol/min/μl hemolymph). The primary [3H]NLPBAN degradation reaction inM. sexta hemolymph was not inhibited by 20 μM leupeptin, 0.1 mM amastatin, 1 mM EDTA, 1 mM EGTA, 1 mM 1,10-phenanthroline, or 2 mM 4-(2-aminoethyl)benzenesulfonyl fluoride; but secondary reactions may have been affected, as some of the inhibitors changed the radio-HPLC profile of the degradation products. It is concluded that hemolymph ofM. sexta andH. zea contains peptidase(s) capable of inactivating circulating PBAN.
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Raina, A. K., and Klun, J. A., Science225 (1984) 531.
Raina, A. K., Jaffe, H., Kempe, T. G., Keim, P., Blacher, R. W., Fales, H. M., Riley, C. T., Klun, J. A., Ridgway, R. L., and Hayes, D. K., Science244 (1989) 796.
Ma, P. W. K., Knipple, D. C., and Roelofs, W. L., Proc. natl Acad. Sci. USA91 (1994) 6506.
Nagasawa, H., Kuniyoshi, H., Arima, R., Kawano, T., Ando, T. and Suzuki, A., Archs Insect Biochem. Physiol.25 (1994) 261.
Masler, E. P., Raina, A. K., Wagner, R. M., and Kochansky, J. P., Insect Biochem. molec. Biol.24 (1994) 829.
Raina, A. K., A. Rev. Ent.38 (1993) 329.
Raina, A. K., and Menn, J. J., Archs Insect Biochem. Physiol.22 (1993) 141.
Raina, A. K., and Kempe, T. G., Insect Biochem.20 (1990) 849.
Matsumoto, S., Kitamura, A., Nagasawa, H., Kataoka, H., Orikasa, C., Mitsui, T., and Suzuki, A., J. Insect Physiol.36 (1990) 427.
Teal, P. E. A., Tumlinson, J. H., and Oberlander, H., Proc. natl Acad. Sci. USA86 (1989) 2488.
Raina, A. K., Rafaeli, A., and Kingan, T. G., J. Insect Physiol.40 (1994) 393.
Quistad, G. B., Adams, M. E., Scarborough, R. M., Carney, R. L., and Schooley, D. A., Life Sci.34 (1984) 569.
Steele, R. W., and Starratt, A. N., Insect Biochem.15 (1985) 511.
Wagner, R. M., Bird, T. G., and Kochansky, J., in: Insect Neurochemistry and Neurophysiology, p. 271. Eds A. B. Borkovec and E. P. Masler. Humana Press, Clifton, NJ 1990.
Fox, A. M., and Reynolds, S. E., Peptides12 (1991) 937.
Rayne, R. C., and O'Shea, M., Insect Biochem. molec. Biol.22 (1992) 25.
Siegert, K. J., and Mordue, W., Insect Biochem. molec. Biol.22 (1992) 657.
Kuniyoshi, H., Nagasawa, H., Ando, T., and Suzuki, A., Insect Biochem. molec. Biol.22 (1992) 399.
Raina, A. K., Kempe, T. G., and Jaffe, H., in: Insect Neuropeptides: Chemistry, Biology, and Action, ACS Symp. Ser., vol. 453, p. 100. Eds J. J. Menn, T. J. Kelly and E. P. Masler. ACS, Washington, DC 1991.
Weirich, G. F., Kochansky, J. P., Masler, E. P., Lusby, W. R., Wagner, R. M., Feldlaufer, M. F., and Svoboda, J. A., Analyt. Biochem.216 (1994) 228.
Weirich, G. F., Feldlaufer, M. F., and Svoboda, J. A., Archs Insect Biochem. Physiol.23 (1993) 199.
Raina, A. K., Klun, J. A., and Stadelbacher, E. A., Ann. ent. Soc. Am.79 (1986) 128.
Peterson, G. L., Analyt. Biochem.83 (1977) 346.
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Weirich, G.F., Kochansky, J.P., Masler, E.P. et al. Degradation of pheromone biosynthesis-activating neuropeptide (PBAN) by hemolymph enzymes of the tobacco hornworm,Manduca sexta, and the corn earworm,Helicoverpa zea. Experientia 51, 961–966 (1995). https://doi.org/10.1007/BF01921748
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DOI: https://doi.org/10.1007/BF01921748