Skip to main content
SpringerLink
Log in

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

  • Abstracts
  • Published:
Cellular and Molecular Life Sciences Aims and scope Submit manuscript

Conclusions

The application of PCR and techniques confirming the specificity of PCR products in routine diagnostic laboratories, requires that the procedures are simple and reproducible. The microtitre plate assay we described for detection of HIV-1 is sensitive, simple, rapid and reproducible. The DEIA test is perfectly compatible with standard enzyme linked immunosorbent assay equipment, permitting the processing of a large number of samples. Moreover, the ability to analyze DNA extracted from dried blood specimens, as demonstrated in this study, allows the long-term storage of blood samples even at elevated temperatures and after transport over long distances.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Mantero, G., Zonaro, A., Albertini, A., Bertolo, P., and Primi, D., Clin. Chem.37 (1991) 422.

    Article  CAS  PubMed  Google Scholar 

  2. Yourno, J., and Conroy, J., J. clin. Microbiol.30 (1992) 2887.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Robert Koch-Institute, Nordufer 20, D-13353, Berlin, Germany

    B. Schweiger, C. Kücherer, C. Fleischer, H. v. Spreckelsen, P. Zablocki-Kaiser & G. Pauli

Authors
  1. B. Schweiger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Kücherer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Fleischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. v. Spreckelsen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. Zablocki-Kaiser
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Pauli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schweiger, B., Kücherer, C., Fleischer, C. et al. PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1. Experientia 52, 308–309 (1996). https://doi.org/10.1007/BF01919526

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01919526

Keywords

Search

Navigation