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Change in cardiac myocyte size distribution in aortic-constricted neonatal rats

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Summary

Regional changes in cardiac myocyte size and population distribution were examined in Sprague Dawley rats receiving an abdominal aortic constriction at five days of age. At specific time intervals post-constriction, hearts were recovered from constricted animals and weight-matched controls and isolated myocytes were obtained from right and left ventricles using retrograde coronary perfusion with collagenase. Cell volume and cardiac myocyte population distribution curves were determined using a Coulter Channelyzer system. Cell length was measured directly using a Bioquant Image Analysis system. Myocyte cross-sectional area was calculated from cell volume/length. By three months of age, heart weight and heart weight-body weight ratio in constricted animals had increased by approximately 115% (p<0.001) in females and 85% (p<0.001) in males compared to controls. Between 15 and 90 days of age, the growth response, as indicated by increased cell volume, was approximately 4× greater in constricted females and 2.5× greater in constricted males compared to corresponding controls. This increase was manifested by a shift in the mean size of the myocyte population to the right and a substantial widening of the distribution. Most of the enlargement was due to increased cross-sectional area, with only a moderate contribution from increased cell length. Significant increases in size were seen in both left and riggt ventricles. By three months of age, a significant interaction was apparent between aortic constriction and sex. The capacity for hypertrophy was greater in the smaller myocytes in female rats of similar age compared to males. The final degree of hypertrophy was similar formale and female rats, possibly indicating a critical upper limit in cell size for cardiac myocytes.

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Author notes

  1. S. E. Campbell Ph.D.

    Present address: Department of Physiology, University of Ottawa, Health Sciences Center, 451 Smyth Road, K1H 8M5, Ottawa, Ontario, Canada

Authors and Affiliations

  1. Department of Physiology, University of Ottawa, Health Sciences Center, 451 Smyth Road, K1H 8M5, Ottawa, Ontario, Canada

    K. Rakusan

  2. Department of Anatomy, University of South Florida, College of Medicine, Tampa, Florida, USA

    A. M. Gerdes

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  1. S. E. Campbell Ph.D.
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  2. K. Rakusan
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  3. A. M. Gerdes
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Additional information

This work was supported by the Ontario Heart and Stroke Foundation (Postdoctoral Research Fellowship to Dr. Campbell and grant to Dr. Rakusan) and Grant RO1 HL30696 from the National Institutes of Health to Dr. Gerdes.

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Campbell, S.E., Rakusan, K. & Gerdes, A.M. Change in cardiac myocyte size distribution in aortic-constricted neonatal rats. Basic Res Cardiol 84, 247–258 (1989). https://doi.org/10.1007/BF01907972

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  • DOI: https://doi.org/10.1007/BF01907972

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