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Inducer effect of Tween 20 permeabilization treatment used for release of stored tropane alkaloids inDatura innoxia Mill. hairy root cultures

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Summary

The effects of Tween 20 as permeabilizing agent on tropane alkaloids fromDatura innoxia Mill. hairy root cultures have been studied. The kinetics of the alkaloid release is detailed and shows three different stages: an initial rapid increase of the alkaloid level within the roots and in the culture medium, followed by a slower but higher increase of the alkaloid concentration in the medium. During this phase, the alkaloid concentration within the roots returned to a lower value. Finally, after a longer time, the quantity of hyoscyamine in the medium decreased significantly with a variable rate. According to the total alkaloid content per flask determinations under different conditions, it is clearly demonstrated that Tween treatment permeabilized the roots, but also acted as an inducer.

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Abbreviations

ACM:

Alkaloid concentration in the culture medium

ACR:

Alkaloid concentration within the roots

ACPF:

Alkaloid concentration per flask

DW:

Dry weight

HPLC:

High pressure liquid chromatopranhv

UV:

Ultra violet

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Authors and Affiliations

  1. Laboratoire Androgenèse et Biotechnologie, Faculté des Sciences, Université de Picardie Jules Verne, 33, rue Saint Len, F-80039, Amiens Cédex 01, France

    Michèle Boitel-Conti, Eric Gontier, Jean-Claude Laberche, Corinne Ducrocq & Brigitte S. Sangwan-Norreel

Authors
  1. Michèle Boitel-Conti
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  2. Eric Gontier
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  3. Jean-Claude Laberche
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  4. Corinne Ducrocq
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  5. Brigitte S. Sangwan-Norreel
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Communicated by W. Barz

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Boitel-Conti, M., Gontier, E., Laberche, JC. et al. Inducer effect of Tween 20 permeabilization treatment used for release of stored tropane alkaloids inDatura innoxia Mill. hairy root cultures. Plant Cell Reports 16, 241–244 (1996). https://doi.org/10.1007/BF01890876

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  • DOI: https://doi.org/10.1007/BF01890876

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