Abstract
A number of factors influencing embryogenesis from isolated microspores of radish (Raphanus sativus) were examined. Of 11 genotypes evaluated, six produced embryos ranging from 8.3 embryos per 105 microspores for ‘Chugoku-ao’ to 0.2 for ‘Tenshun’, but five genotypes were not responsive. An initial culture period at elevated temperature before incubation at 25°C was essential for induction of microspore embryogenesis. However, the optimum period of the treatment varied among genotypes and/or experiments. Bud size also influenced microspore embryogenesis. Though optimum bud size was different between genotypes, the microspore populations represented in these buds contained uninucleate and binucleate microspores. Selection of embryogenic microspores using percoll density gradient resulted in up to 1.3-fold increase of embryo yield. Though almost all embryos failed to develop directly into plantlets, plants were obtained by multiple subcultures. The regenerated plants had hyperploid chromosome numbers.
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Abbreviations
- BA:
-
6-benzylaminopurine
- IAA:
-
indole-3-acetic acid
References
Burnett L, Yarrow S, Huang B (1992) Plant Cell Rep 11: 215–218
Chuong PV, Beversdorf WD (1985) Plant Sci 39: 219–226
Chuong PV, Deslauriers C, Kott KS, Beversdorf WD (1988) Can J Bot 66: 1653–1657
Duijs JG, Voorrips RE, Visser DL, Custers JBM (1992) Euphytica 60: 45–55
Fan Z, Holbrook L, Keller WA (1987) Proc 7th Internat Rapeseed Congr, Poland, pp 92–96
Fan Z, Armstrong KC, Keller WA (1988) Protoplasma 147: 191–199
Ferrie AMR, Epp DJ, Keller WA (1995) Plant Cell Rep 14: 580–584
Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158
Gland A, Lichter R, Schweiger H-G (1988) J Plant Physiol 132: 613–617
Hansen M, Svinnset (1993) Plant Cell Rep 12: 496–500
Jeong WJ, Min Sr, Liu JR (1995) Plant Cell Rep 14: 648–651
Lichter R (1982) Z Pflanzenphysiol 105: 427–434
Lichter R (1989) Plant Breeding 103: 119–123
Matsubara S, Hegazi HH (1990) HortScience 25: 1286–1288
Murakami T, Ono Y, Takahata Y (1995) Plant Tissue Cult Lett 12: 321–323
Ohkawa Y, Bevis E, Keller WA (1988) Cruciferae Newslett 13: 75
Pechan PM, Keller WA (1988) Physiol Plant 74: 377–384
Prakash S, Hinata K (1980) Opera Botanica 55: 1–57
Sato T, Nishio T, Hirai M (1989) Plant Cell Rep 8: 486–488
Siebel J, Pauls KP (1989) Theor Appl Genet 78: 473–479
Takahata Y (1990) Euphytica 46: 259–264
Takahata Y, Keller WA (1991) Plant Sci 74: 235–242
Takahata Y, Takani Y, Kaizuma N (1993) Plant Tissue Cult Lett 10: 49–53
Telmer CA, Simmonds DH, Newcomb W (1992) Physiol Plant 84: 417–424
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Communicated by G. C. Phillips
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Takahata, Y., Komatsu, H. & Kaizuma, N. Microspore culture of radish (Raphanus sativus L.): influence of genotype and culture conditions on embryogenesis. Plant Cell Reports 16, 163–166 (1996). https://doi.org/10.1007/BF01890859
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DOI: https://doi.org/10.1007/BF01890859