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The early appearance of fibronectin in the course of metastatic tumor growth in lymph nodes

  • Original Papers
  • Clinical Oncology or Epidemiology
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Summary

In order to study structural changes in the lymph node architecture in the course of metastatic involvent by carcinoma, 70 axillary lymph nodes of 45 female patients affected by breast carcinoma were examined. The follwing growth pattern of the metastasis was observed: (1) Involvement of vasa afferentia and (marginal) sinuses; (2) lysis of sinus wall and infiltration, causing replacement and lysis of reticulin fibres; (3) appearance of fibrillary fibronectin (FN) as the first noticeable sign of tumor stroma; (4) immigration of fibroblasts along these fibres, appearance of argyrophilic fibres codistributed with fibrillary FN; (5) formation of the complete tumor stroma, neovessels included. The appearance of FN is thus the first step of stroma formation and is interpreted as an “in situ” precipitation of soluble plasma FN.

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References

  • Ali IU, Hynes RO (1978) Effects of LETS glycoprotein on cell motility. Cell 14:429–446

    PubMed  Google Scholar 

  • Alitalo K, Hovi T, Vahen A (1980) Fibronectin is produced by human macrophages. J Exp Med 151:602–613

    PubMed  Google Scholar 

  • d'Ardenne AJ, McGee JOD (1984) Fibronectin in disease. J Pathol 142:235–251

    PubMed  Google Scholar 

  • Asch BB, Kama BR, Burstein NA (1981) Interactions of normal, dysplastic, and malignant mammary epithelial cells with fibronectin in vivo and in vitro. Cancer Res 41:2115–2125

    PubMed  Google Scholar 

  • Atherton BT, Hynes RO (1981) A difference between plasma and cellular fibronectins located with monoclonal antibodies. Cell 25:133–141

    PubMed  Google Scholar 

  • Birdwell CR, Cosporadowicz D, Nicolson GL (1978) Identification, localization and role of fibronectin in cultured bovine endothelial cells. Proc Natl Acad Sci USA 75:3273–3277

    PubMed  Google Scholar 

  • Bussolati G, Gugliotta P (1983) Nonspecific staining of mast cells by avidin-biotin-peroxidase complexes (ABC). J Histochem Cytochem 31, 12:1419–1421

    Google Scholar 

  • Chen LB, Gallimore PH, McDougall JK (1976) Correlation between tumor induction and the large external transformation sensitive protein on the cell surface. Proc Natl Acad Sci USA 73:3570–3574

    PubMed  Google Scholar 

  • Clark RAF, Quinn JH, Winn HJ, Lanigan JM, Dellapelle P, Colvin RV (1982a) Fibronectin is produced by blood vessels in response to injury. J Exp Med 156:646–651

    PubMed  Google Scholar 

  • Clark RAF, Dellapelle P, Mansean E, Lanigan JM, Dvorak HF, Colvin RB (1982b) Blood vessel fibronectin increases in conjunction with endothelial cell proliferation and capillary in growth during wound healing. J Invest Dermatol 79:269–276

    PubMed  Google Scholar 

  • Clark RAF, Lanigan JM, Dellapelle P, Manseau E, Dvorak HF, Colvin RB (1982c) Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization. J Invest Dermatol 79:264–269

    PubMed  Google Scholar 

  • Der CJ, Stanbridge E (1978) Lack of correlation between the decreased expression of cell surface LETS protein and tumorigenicity in human cell hybrids. Cell 15:1241–1251

    PubMed  Google Scholar 

  • Gallimore PH, MacDougall JK, Chen LB (1979) Malignant behaviour of three adenovirus 2-transformed brain cell lines and their methylcellulose selected subclones. Int J Cancer 24:477–484

    PubMed  Google Scholar 

  • Grinnell F, Feld M, Minter D (1980) Fibroblast adhesion to fibrinogen and fibrin substrata: Requirement of cold-insoluble globulin (plasma fibronectin). Cell 19:517–525

    PubMed  Google Scholar 

  • Grinnell F, Billingham RE, Burgess L (1981) Distribution of fibronectin during wound healing in vivo. J Invest Dermatol 76:181–189

    PubMed  Google Scholar 

  • Hayman EG, Engvall E, Ruoslahti E (1981) Concomitant loss of cell surface fibronectin and laminin from transformed rat kidney cells. J Cell Biol 88:352–257

    PubMed  Google Scholar 

  • Hörmann H (1982) Fibronectin-mediator between cells and connective tissue. Klin Wochenschr 60:1265–1277

    PubMed  Google Scholar 

  • Hynes RO, Yamada KM (1982) Fibronectins: multifunctional modular glycoproteins. J Cell Biol 95:369–377

    PubMed  Google Scholar 

  • Jaffe EA, Mosher DF (1978) Synthesis of fibronectin by cultured human endothelial cells. J Exp Med 147:1779–1791

    PubMed  Google Scholar 

  • Johansson SK, Rubin M, Hook T, Ahlgren T, Seljelin R (1979) In vitro biosynthesis of cold insoluble globulin (fibronectin) by mouse peritoneal macrophages. Fed Eur Biochem Soc Lett 105:313–316

    Google Scholar 

  • Jones PA, Laug WE, Gardner A, Nye CA, Fink LM, Benedict WF (1976) In vitro correlates of transformation in C3H/10 t-1/2 clone 8 mouse cells. Cancer Res 36:2863–2867

    PubMed  Google Scholar 

  • Kahn P, Shin SI (1979) Cellular tumorigenicity in nude mice: Test of association among loss of cell surface fibronectin, anchorage independence and tumour forming ability. J Cell Biol 82:1–16

    PubMed  Google Scholar 

  • Klebe RJ (1974) Isolation of a collagen-dependent cell attachment factor. Nature 250:248–251

    PubMed  Google Scholar 

  • Kubo M, Norris DA, Howell SE, Ryan SR, Clark RAF (1984) Human keratinocytes synthesize, secrete, and deposite fibronectin in the pericellular matrix. J Invest Dermatol 82, 6:580–586

    PubMed  Google Scholar 

  • Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51

    PubMed  Google Scholar 

  • Labat-Robert J, Birembaut P, Adnet JJ, Mercantini F, Robert L (1980) Loss of fibronectin in human breast cancer. Cell Biol Int Rep 4:609–616

    PubMed  Google Scholar 

  • Linder E, Stenman S, Lehto P, Vaheri A (1978) Distribution of fibronectin in human tissues and relationship to other connective tissue components. Ann NY Acad Sci 312:151–159

    PubMed  Google Scholar 

  • Möller P, Feichter GE, Fritze D, Haag D, Schüle B (1982) J-chainproducing immunoblastic lymphoma in a case of Richter's syndrome. Virchows Arch Pathol Anat 396:213–224

    PubMed  Google Scholar 

  • Möller P, Aststätter H, Butzengeiger N, Schüle B (1983) The distribution of fibronectin in lymph nodes infiltrated by Hodgkin's disease. Virchows Arch Pathol Anat 400:319–329

    Google Scholar 

  • Neri A, Nicolson GL (1981) Phenotypic drift of metastatic and cell surface properties of mammary adenocarcinoma cell clones during growth in vitro. Int J Cancer 28:731–738

    PubMed  Google Scholar 

  • Neri A, Ruoslahti E, Nicolson GL (1981) Distribution of fibronectin on clonal cell lines of a rat mammary adenocarcinoma growing in vitro and in vivo at primary and metastatic sites. Cancer Res 41:5082–5095

    PubMed  Google Scholar 

  • Niemczuk P, Perkins RM, Talbot IC, Critchley DR (1982) Lack of correlation between metastasis of human rectal carcinoma and the absence of stromal fibronectin. Br J Cancer 45:500–505

    PubMed  Google Scholar 

  • Pearlstein E (1978) Substrate activation of cell adhesion factor as a prerequisite of cell attachment. Int J Cancer 22:32–35

    PubMed  Google Scholar 

  • Pearlstein E, Gold LI (1978) High-molecular-weight glycoproteins as a mediator of cellular adhesion. Ann NY Acad Sci 312:278–292

    PubMed  Google Scholar 

  • Pouyssegur J, Willingham M, Pastan I (1977) Role of cell surface carbohydrates and proteins in cell behavior: Studies on the biochemical reversion of an N-acetyl glucosamine deficient fibroblast mutant. Proc Natl Acad Sci USA 74:243–247

    PubMed  Google Scholar 

  • Ruoslahti E, Vaheri A (1974) Novel human serum protein from fibroblast plasma membrane. Nature 248:789–791

    PubMed  Google Scholar 

  • Schwartz CE, Ruoslahti E (1983) Concurrent modulation of cell surface fibronectin and adhesion of fibronectin in hepatoma cells. Exp Cell Res 143:456–461

    PubMed  Google Scholar 

  • Smith HS, Riggs JL, Mosesson MW (1979) Production of fibronectin by human epithelial cells in culture. Cancer Res 39:4138–4144

    PubMed  Google Scholar 

  • Stenman S, Vaheri A (1978) Distribution of a major connective tissue protein, fibronectin, in normal tissue. J Exp Med 147:1054–1064

    PubMed  Google Scholar 

  • Stenman S, Vaheri A (1981) Fibronectin in human solid tumours. Int J Cancer 27:427–435

    PubMed  Google Scholar 

  • Taylor-Papadimitriou J, Burchell J, Hurst J (1981) Production of fibronectin by normal and malignant human mammary epithelial cells. Cancer Res 41:2491–2500

    PubMed  Google Scholar 

  • Vaheri A, Ruoslahti E (1975) Fibroblast surface antigen produced but not retained by virus-transformed human cells. J Exp Med 142:530–535

    PubMed  Google Scholar 

  • Vaheri A, Ruoslahti E, Westermak B, Ponten J (1976) A common cell type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells. J Exp Med 143:64–69

    PubMed  Google Scholar 

  • Voss B, Allah S, Rauterberg J, Ullrich K, Gieselmann V, von Fjgura K (1979) Primary cultures of rat hepatocytes synthesize fibronectin. Biochem Biophys Res Commun 90:1348–1354

    PubMed  Google Scholar 

  • Vuento M, Vartio T, Saraste M, von Bonsdorff CH, Vaheri A (1980) Spontaneous and polyamine-induced formation of filamentous polymers from soluble fibronectin. Eur J Biochem 105:33–42

    PubMed  Google Scholar 

  • Williams EC, Janmey PA, Ferry JD, Mosher DF (1982) Conformational states of fibronectin. J Biol Chem 257, 24:14973–14978

    PubMed  Google Scholar 

  • Yamada KM, Kennedy DW (1979) Fibroblast cellular and plasma fibronectins are similar but not identical. J Cell Biol 80:492–498

    PubMed  Google Scholar 

  • Yang NS, Kirkland W, Jorgensen T, Furmanski P (1980) Absence of fibronectin and presence of plasminogen activator in both normal and malignant human mammary epithelial cells in culture. J Bell Biol 84:120–130

    Google Scholar 

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  1. Institut für Allgemeine Pathologie und pathologische Anatomie der Universität Heidelberg, Im Neuenheimer Feld 220/221, D-6900, Heidelberg 1, Federal Republic of Germany

    Dietrich Lorke & Peter Möller

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  1. Dietrich Lorke
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  2. Peter Möller
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Lorke, D., Möller, P. The early appearance of fibronectin in the course of metastatic tumor growth in lymph nodes. J Cancer Res Clin Oncol 109, 65–71 (1985). https://doi.org/10.1007/BF01884257

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  • DOI: https://doi.org/10.1007/BF01884257

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