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Monolayer black membranes from bipolar lipids of archaebacteria and their temperature-induced structural changes

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Summary

The membrane ofCaldariella acidophila, an extreme thermophilic archaebacterium, is characterized by unusual bipolar complex lipids. They consist of two nonequivalent polar heads, linked by a C40 alkylic component. The molecular organization of these lipids in the plasma membrane is still a matter of study. In this paper, we present current-voltage measurements on artificial bipolar lipid membranes, indicating that molecules are indeed organized as a covalently bound bilayer, in which each molecule is completely stretched and spans its entire thickness. Furthermore, conformational transitions of these artificial membranes (which could be formed only above 70°C from a lipid/squalene dispersion) are analyzed in the 80 to 15°C temperature range. Abrupt variations in capacitance and valinomycin-induced conductance seem to indicate the occurrence of at least two structural changes. Measurements are also extended to different solvent systems. Results are consistent with the picture of a monolayer bipolar lipid membrane in which few solvent molecules align themselves parallel to the lipophilic chains. The amount of solvent as well as the temperature at which conformational transitions occur, depend on the solvent system in which the lipid is dispersed.

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Authors and Affiliations

  1. Istituto di Cibernetica e Biofisica, CNR, 16032, Camogli, Italy

    Alessandra Gliozzi

  2. Istituto di Scienze Fisiche dell'Università di Genova, 16132, Italy

    Ranieri Rolandi

  3. Istituto per la Chimica di Molecole di Interesse Biologico, Arco Felice (NA), 80072, Italy

    Mario De Rosa & Agata Gambacorta

Authors
  1. Alessandra Gliozzi
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  2. Ranieri Rolandi
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  3. Mario De Rosa
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  4. Agata Gambacorta
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Gliozzi, A., Rolandi, R., De Rosa, M. et al. Monolayer black membranes from bipolar lipids of archaebacteria and their temperature-induced structural changes. J. Membrain Biol. 75, 45–56 (1983). https://doi.org/10.1007/BF01870798

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  • DOI: https://doi.org/10.1007/BF01870798

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