Summary
Suspensions of log phase cells ofRhodospirillum rubrum at pH 5.5 show a light-induced decrease in the pH of the medium which is reversed during the subsequent dark period. The velocity and magnitude of the pH change were the same whether the cells were bubbled with air, CO2-free air or N2 during experimentation. The pH response is temperature dependent. Phenazine methyl sulfate (PMS) at concentrations above 0.05mm stimulates the light-induced pH change. PMS at 1mm gives a 2-fold increase in the initial rate upon illumination and a 1.5-fold increase in the total change in pH after 2 min of illumination. The inhibition of the proton transport by 10 μg/ml antimycin A or 20 μm 2-n-heptyl-4-hydroxyquinoline-N-oxide can be partially relieved by PMS. However, inhibition of the light-induced proton transport with 0.5mm 2,4-dinitrophenol or 3 μm carbonylcyanide-m-chlorophenylhydrazone (CCCP) cannot be overcome by addition of PMS. Valinomycin, at a concentration of 3 μm, caused a slight stimulation of the light-induced proton transport in the presence of 200mm KCl. The inhibition of proton transport by 3 μm CCCP was partially relieved with 3 μm valinomycin in the presence of 200mm KCl, but the antibiotic was without effect when the cells were suspended in 200mm NaCl. The results are discussed in terms of current theories of the action of PMS, antimycin A, valinomycin, and uncouplers on the light-induced electron flow and photophosphorylation inR. rubrum.
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Baltscheffsky, H. 1960. Flavin nucleotides and light-induced phosphorylation in cell-free extracts ofRhodospirillum rubrum.Biochim. Biophys. Acta 40:1.
— Arwidsson, B. 1962. Evidence for two phosphorylating sites in bacterial cyclic photophosphorylation.Biochim. Biophys. Acta 65:425.
—, Baltscheffsky, M. 1958. On light-induced phosphorylation inRhodospirillum rubrum.Acta Chem. Scand. 12:1333.
Carafoli, E., Rossi, C. S. 1967. The effect of dinitrophenol on the permeability of the mitochondrial membrane.Biochem. Biophys. Res. Commun. 29:153.
Chappell, J. B., Haarhoff, K. N. 1967. The penetration of the mitochondrial membrane by anions and cations.In: Biochemistry of Mitochondria. E. C. Slater, Z. Kaniuga, and L. Wojtczak, editors. p. 75. Academic Press, New York.
Cohen-Bazire, G., Sistrom, W., Stanier, R. Y. 1957. Kinetic studies of pigment synthesis by non-sulfur purple bacteria.J. Cell. Comp. Physiol. 49:25.
Dilley, R. A., Vernon, L. P. 1965. Ion and water transport processes related to the light dependent shrinkage of spinach chloroplasts.Arch. Biochem. Biophys. 111:365.
Edwards, G. E., Bovell, C. R. 1969. Characteristics of a light-dependent proton transport in cells ofRhodospirillum rubrum.Biochim. Biophys. Acta 172:126.
Geller, D. M., Lipmann, F. 1960. Photophosphorylation in extracts ofRhodospirillum rubrum.J. Biol. Chem. 235:2478.
Hopfer, U., Lehinger, A. L., Thompson, T. E. 1968. Proton conductance across phospholipid bilayer membranes induced by uncoupling agents for oxidative phosphorylation.Proc. Nat. Acad. Sci. 59:484.
Horio, T., Kamen, M. D. 1962. Optimal oxidation-reduction potentials and endogenous co-factors in bacterial photophosphorylation.Biochemistry 1:144.
Jackson, J. B., Crofts, A. R., von Stedingk, L. V. 1968. Ion transport induced by light and antibiotics in chromatophores fromRhodospirillum rubrum.Europ. J. Biochem. 6:41.
Jagendorf, A. T. 1967. Acid-base transitions and phosphorylation by chloroplasts.Fed. Proc. 26:1361.
King, T. E. 1963. Reconstitution of respiratory chain enzyme systems, XI. Use of artificial electron acceptors in the assay of succinate-dehydrogenating enzymes.J. Biol. Chem. 238:4032.
Mitchell, P. 1966. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation.Biol. Rev. Cambridge Phil. Soc. 41:445.
— 1967. Proton-translocation phosphorylation in mitochondria, chloroplasts, and bacteria: natural fuel cells and solar cells.Fed. Proc. 26:1370.
Moore, C., Pressman, B. C. 1964. Mechanism of action of valinomycin on mitochondria.Biochem. Biophys. Res. Commun. 15:562.
Nishimura, M. 1962. Studies on bacterial photophosphorylation. II. Effects of reagents and temperature on light-induced and dark phases of photophosphorylation inRhodospirillum rubrum chromatophores.Biochim. Biophys. Acta 57:96.
— 1963. Studies on the electron-transfer systems in photosynthetic bacteria. II. The effect of heptylhydrox-quinoline-N-oxide and antimycin A on the photosynthetic and respiratory electron-transfer systems.Biochim. Biophys. Acta 66:17.
— 1963. Light-induced and dark steps of bacterial photophosphorylation.In: Bacterial Photosynthesis. A. San Pietro and L. P. Vernon, editors. p. 201. The Antioch Press, Yellow Springs, Ohio.
Okayama, S., Yamamoto, N., Nishikawa, K., Horio, T. 1968. Roles of ubiquinone-10 and rhodoquinone in photosynthetic formation of adenosine triphosphate by chromatophores fromRhodospirillum rubrum.J. Biol. Chem. 243:2995.
Parson, W. W. 1967. Flash-induced absorbance changes inRhodospirillum rubrum chromatophores.Biochim. Biophys. Acta 131:154.
Pavlasova, E., Harold, F. M. 1969. Energy coupling in the transport of B-Galactosides byEscherichia coli: Effect of proton conductors.J. Bacteriol. 98:198.
Polya, G. M., Jagendorf, A. T. 1969. Light-induced change in the buffer capacity of spinach chloroplast suspensions.Biochem. Biophys. Res. Commun. 36:696.
Sato, H., Takahashi, K., Kikuchi, G. 1966. Inhibition studies of photophosphorylation byRhodospirillum rubrum chromatophores with particular concerns to antimycin-resistant photophosphorylation in the presence of artificial electron carriers.Biochim. Biophys. Acta 112:8.
Scholes, P., Mitchell, P., Moyle, J. 1969. The polarity of proton translocation in some photosynthetic microorganisms.Europ. J. Biochem. 8:450.
Schuldiner, S., Ohad, I. 1969. Biogenesis of chloroplast membranes. III. Light-dependent induction of proton pump activity in whole cells and its correlation to cytochromef photooxidation during greening of aChlamydomonas reinhardti mutant (y-I).Biochim. Biophys. Acta 180:165.
Shavit, N., Thore, A., Keister, D. L., San Pietro, A. 1968. Inhibition by nigericin of the light-induced pH change inRhodospirillum rubrum chromatophores.Biochemistry 59:917.
Stedingk, L. V. von. 1967. Light-induced reversible pH changes in chromatophores fromRhodospirillum rubrum.Arch. Biochem. Biophys. 120:537.
— Baltscheffsky, N. 1966. The light-induced, reversible pH change in chromatophores fromRhodospirillum rubrum.Arch. Biochem. Biophys. 117:400.
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Edwards, G.E., Bovell, C.R. Action of phenazine methyl sulfate, inhibitors, and uncouplers on the light-induced proton transport by cells ofRhodospirillum rubrum . J. Membrain Biol. 2, 95–107 (1970). https://doi.org/10.1007/BF01869853
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DOI: https://doi.org/10.1007/BF01869853