Abstract
An overview of the principles of the polymerase chain reaction, ligase chain reaction, self-sustained sequence replication and Qβ replicase is given. The application of these methods for the diagnosis of veterinary infectious and hereditary diseases as well as for other diagnostic purposes is discussed and comprehensive tables of reported assays are provided. Specific areas where these DNA-based amplification methods provide substantial advantages over traditional approaches are also highlighted. With regard to PCR-based assays for the detection of viral pathogens, this article is an update of a previous review by Belák and Ballagi-Pordány (1993).
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Abbreviations
- 3SR:
-
self-sustained sequence replication
- BHV:
-
bovine herpesvirus
- BLAD:
-
bovine leukocyte adhesion deficiency
- cDNA:
-
complementary DNA
- CFU:
-
colony-forming units
- EHV:
-
equine herpesvirus
- ELISA:
-
enzyme-linked immunosorbent assay
- FHV:
-
feline herpesvirus
- LCR:
-
ligase chain reaction
- PCR:
-
polymerase chain reaction
- PRV:
-
pseudorabies virus
- RAPD:
-
random amplified polymorphic DNA
- RFLP:
-
restriction fragment length polymorphism
- RT:
-
reverse transcriptase
- rRNA:
-
ribosomal RNA
- wt:
-
wild-type
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These two authors contributed equally to this review and are listed in alphabetical order.
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Pfeffer, M., Wiedmann, M. & Batt, C.A. Applications of DNA amplification techniques in veterinary diagnostics. Vet Res Commun 19, 375–407 (1995). https://doi.org/10.1007/BF01839319
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DOI: https://doi.org/10.1007/BF01839319